TY - JOUR
T1 - Method development and validation of a high-performance liquid chromatographic method for tramadol in human plasma using liquid-liquid extraction
AU - Gan, S. H.
AU - Ismail, R.
AU - Wan Adnan, W. A.
AU - Wan, Z.
N1 - Funding Information:
We would like to thank Tengku Azaha for his skilful technical assistance in preparing the samples and operating the HPLC system, Lukmi Ismail for his invaluable advice, Grunenthal GmbH (Germany) for providing drug standards and metabolite and the Malaysian Ministry of Science and Technology for financial support under IRPA (Intensified Research in Priority Area) research grants.
PY - 2002/5/25
Y1 - 2002/5/25
N2 - An HPLC system using a simple liquid-liquid extraction and HPLC with UV detection has been validated to determine tramadol concentration in human plasma. The method developed was selective and linear for concentrations ranging from 10 to 2000 ng/ml with average recovery of 98.63%. The limit of quantitation (LOQ) was 10 ng/ml and the percentage recovery of the internal standard phenacetin was 76.51%. The intra-day accuracy ranged from 87.55 to 105.99% and the inter-day accuracy, 93.44 to 98.43% for tramadol. Good precision (5.32 and 6.67% for intra- and inter-day, respectively) was obtained at LOQ. The method has been applied to determine tramadol concentrations in human plasma samples for a pharmacokinetic study.
AB - An HPLC system using a simple liquid-liquid extraction and HPLC with UV detection has been validated to determine tramadol concentration in human plasma. The method developed was selective and linear for concentrations ranging from 10 to 2000 ng/ml with average recovery of 98.63%. The limit of quantitation (LOQ) was 10 ng/ml and the percentage recovery of the internal standard phenacetin was 76.51%. The intra-day accuracy ranged from 87.55 to 105.99% and the inter-day accuracy, 93.44 to 98.43% for tramadol. Good precision (5.32 and 6.67% for intra- and inter-day, respectively) was obtained at LOQ. The method has been applied to determine tramadol concentrations in human plasma samples for a pharmacokinetic study.
KW - Tramadol
UR - http://www.scopus.com/inward/record.url?scp=0037172356&partnerID=8YFLogxK
U2 - 10.1016/S1570-0232(02)00065-X
DO - 10.1016/S1570-0232(02)00065-X
M3 - Article
C2 - 12016023
AN - SCOPUS:0037172356
SN - 1570-0232
VL - 772
SP - 123
EP - 129
JO - Journal of Chromatography B
JF - Journal of Chromatography B
IS - 1
ER -