Zwitterionic modification of ultrasmall iron oxide nanoparticles for reduced protein corona formation

Karina Pombo-García, Carmen L. Rühl, Raymond Lam, Jose A Barreto, Ching-Seng Ang, Peter J Scammells, Peter Comba, Leone Spiccia, Bim Graham, Tanmaya Joshi, Holger Stephan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Polyacrylic-acid-coated ultra-small super-paramagnetic iron oxide nanoparticles were surface-modified with low-molecular-weight sulfobetaines or 3-(diethylamino)propylamine in order to generate nanoparticles with zwitterionic character (ZW-NPs). The ZW-NPs proved highly resistant to serum protein corona formation invitro, as revealed by atomic force microscopy, SDS-PAGE and proteomics analysis, and exhibited low cytotoxicity towards A431 and HEK293 cells. The presence of unreacted carboxylic acid groups enabled additional functionalization with fluorescent (Cy5) and radioactive [64Cu-dmptacn; dmptacn=1,4-bis(2-pyridinylmethyl)-1,4,7-triazacyclononane] moieties. Overall, the ZW-NPs represent promising platforms for the development of new multimodal diagnostic/therapeutic agents possessing "stealth" properties.

Original languageEnglish
Pages (from-to)638–646
Number of pages9
JournalChemPlusChem
Volume82
Issue number4
DOIs
Publication statusPublished - 2017

Keywords

  • Iron oxide nanoparticles
  • Multimodal imaging
  • Protein corona
  • Proteomics
  • Zwitterions

Cite this

Pombo-García, K., Rühl, C. L., Lam, R., Barreto, J. A., Ang, C-S., Scammells, P. J., ... Stephan, H. (2017). Zwitterionic modification of ultrasmall iron oxide nanoparticles for reduced protein corona formation. ChemPlusChem, 82(4), 638–646. https://doi.org/10.1002/cplu.201700052
Pombo-García, Karina ; Rühl, Carmen L. ; Lam, Raymond ; Barreto, Jose A ; Ang, Ching-Seng ; Scammells, Peter J ; Comba, Peter ; Spiccia, Leone ; Graham, Bim ; Joshi, Tanmaya ; Stephan, Holger. / Zwitterionic modification of ultrasmall iron oxide nanoparticles for reduced protein corona formation. In: ChemPlusChem. 2017 ; Vol. 82, No. 4. pp. 638–646.
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Pombo-García, K, Rühl, CL, Lam, R, Barreto, JA, Ang, C-S, Scammells, PJ, Comba, P, Spiccia, L, Graham, B, Joshi, T & Stephan, H 2017, 'Zwitterionic modification of ultrasmall iron oxide nanoparticles for reduced protein corona formation' ChemPlusChem, vol. 82, no. 4, pp. 638–646. https://doi.org/10.1002/cplu.201700052

Zwitterionic modification of ultrasmall iron oxide nanoparticles for reduced protein corona formation. / Pombo-García, Karina; Rühl, Carmen L.; Lam, Raymond; Barreto, Jose A; Ang, Ching-Seng; Scammells, Peter J; Comba, Peter; Spiccia, Leone; Graham, Bim; Joshi, Tanmaya; Stephan, Holger.

In: ChemPlusChem, Vol. 82, No. 4, 2017, p. 638–646.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Zwitterionic modification of ultrasmall iron oxide nanoparticles for reduced protein corona formation

AU - Pombo-García, Karina

AU - Rühl, Carmen L.

AU - Lam, Raymond

AU - Barreto, Jose A

AU - Ang, Ching-Seng

AU - Scammells, Peter J

AU - Comba, Peter

AU - Spiccia, Leone

AU - Graham, Bim

AU - Joshi, Tanmaya

AU - Stephan, Holger

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