Zoonotic transfer of Clostridium difficile harboring antimicrobial resistance between farm animals and humans

C. W. Knetsch, N. Kumar, S. C. Forster, T. R. Connor, H. P. Browne, C. Harmanus, I. M. Sanders, S. R. Harris, L. Turner, T. Morris, M. Perry, F. Miyajima, P. Roberts, M. Pirmohamed, J. G. Songer, J. S. Weese, A. Indra, J. Corver, M. Rupnik, B. W. Wren & 3 others T. V. Riley, E. J. Kuijper, T. D. Lawley

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)

Abstract

The emergence of Clostridium difficile as a significant human diarrheal pathogen is associated with the production of highly transmissible spores and the acquisition of antimicrobial resistance genes (ARGs) and virulence factors. Unlike the hospital-associated C. difficile RT027 lineage, the community-associated C. difficile RT078 lineage is isolated from both humans and farm animals; however, the geographical population structure and transmission networks remain unknown. Here, we applied whole-genome phylogenetic analysis of 248 C. difficile RT078 strains from 22 countries. Our results demonstrate limited geographical clustering for C. difficile RT078 and extensive coclustering of human and animal strains, thereby revealing a highly linked intercontinental transmission network between humans and animals. Comparative whole-genome analysis reveals indistinguishable accessory genomes between human and animal strains and a variety of antimicrobial resistance genes in the pangenome of C. difficile RT078. Thus, bidirectional spread of C. difficile RT078 between farm animals and humans may represent an unappreciated route disseminating antimicrobial resistance genes between humans and animals. These results highlight the importance of the “One Health” concept to monitor infectious disease emergence and the dissemination of antimicrobial resistance genes.

Original languageEnglish
Article numbere01384-17
Number of pages8
JournalJournal of Clinical Microbiology
Volume56
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Keywords

  • Accessory genome
  • Antibiotic resistance
  • Clostridium difficile
  • Intercontinental transmission
  • Interhost transmission
  • One health concept
  • RT078

Cite this

Knetsch, C. W. ; Kumar, N. ; Forster, S. C. ; Connor, T. R. ; Browne, H. P. ; Harmanus, C. ; Sanders, I. M. ; Harris, S. R. ; Turner, L. ; Morris, T. ; Perry, M. ; Miyajima, F. ; Roberts, P. ; Pirmohamed, M. ; Songer, J. G. ; Weese, J. S. ; Indra, A. ; Corver, J. ; Rupnik, M. ; Wren, B. W. ; Riley, T. V. ; Kuijper, E. J. ; Lawley, T. D. / Zoonotic transfer of Clostridium difficile harboring antimicrobial resistance between farm animals and humans. In: Journal of Clinical Microbiology. 2018 ; Vol. 56, No. 3.
@article{820df1a690e741c7bc23c25bb6184b1e,
title = "Zoonotic transfer of Clostridium difficile harboring antimicrobial resistance between farm animals and humans",
abstract = "The emergence of Clostridium difficile as a significant human diarrheal pathogen is associated with the production of highly transmissible spores and the acquisition of antimicrobial resistance genes (ARGs) and virulence factors. Unlike the hospital-associated C. difficile RT027 lineage, the community-associated C. difficile RT078 lineage is isolated from both humans and farm animals; however, the geographical population structure and transmission networks remain unknown. Here, we applied whole-genome phylogenetic analysis of 248 C. difficile RT078 strains from 22 countries. Our results demonstrate limited geographical clustering for C. difficile RT078 and extensive coclustering of human and animal strains, thereby revealing a highly linked intercontinental transmission network between humans and animals. Comparative whole-genome analysis reveals indistinguishable accessory genomes between human and animal strains and a variety of antimicrobial resistance genes in the pangenome of C. difficile RT078. Thus, bidirectional spread of C. difficile RT078 between farm animals and humans may represent an unappreciated route disseminating antimicrobial resistance genes between humans and animals. These results highlight the importance of the “One Health” concept to monitor infectious disease emergence and the dissemination of antimicrobial resistance genes.",
keywords = "Accessory genome, Antibiotic resistance, Clostridium difficile, Intercontinental transmission, Interhost transmission, One health concept, RT078",
author = "Knetsch, {C. W.} and N. Kumar and Forster, {S. C.} and Connor, {T. R.} and Browne, {H. P.} and C. Harmanus and Sanders, {I. M.} and Harris, {S. R.} and L. Turner and T. Morris and M. Perry and F. Miyajima and P. Roberts and M. Pirmohamed and Songer, {J. G.} and Weese, {J. S.} and A. Indra and J. Corver and M. Rupnik and Wren, {B. W.} and Riley, {T. V.} and Kuijper, {E. J.} and Lawley, {T. D.}",
year = "2018",
month = "3",
day = "1",
doi = "10.1128/JCM.01384-17",
language = "English",
volume = "56",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",

}

Knetsch, CW, Kumar, N, Forster, SC, Connor, TR, Browne, HP, Harmanus, C, Sanders, IM, Harris, SR, Turner, L, Morris, T, Perry, M, Miyajima, F, Roberts, P, Pirmohamed, M, Songer, JG, Weese, JS, Indra, A, Corver, J, Rupnik, M, Wren, BW, Riley, TV, Kuijper, EJ & Lawley, TD 2018, 'Zoonotic transfer of Clostridium difficile harboring antimicrobial resistance between farm animals and humans', Journal of Clinical Microbiology, vol. 56, no. 3, e01384-17. https://doi.org/10.1128/JCM.01384-17

Zoonotic transfer of Clostridium difficile harboring antimicrobial resistance between farm animals and humans. / Knetsch, C. W.; Kumar, N.; Forster, S. C.; Connor, T. R.; Browne, H. P.; Harmanus, C.; Sanders, I. M.; Harris, S. R.; Turner, L.; Morris, T.; Perry, M.; Miyajima, F.; Roberts, P.; Pirmohamed, M.; Songer, J. G.; Weese, J. S.; Indra, A.; Corver, J.; Rupnik, M.; Wren, B. W.; Riley, T. V.; Kuijper, E. J.; Lawley, T. D.

In: Journal of Clinical Microbiology, Vol. 56, No. 3, e01384-17, 01.03.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Zoonotic transfer of Clostridium difficile harboring antimicrobial resistance between farm animals and humans

AU - Knetsch, C. W.

AU - Kumar, N.

AU - Forster, S. C.

AU - Connor, T. R.

AU - Browne, H. P.

AU - Harmanus, C.

AU - Sanders, I. M.

AU - Harris, S. R.

AU - Turner, L.

AU - Morris, T.

AU - Perry, M.

AU - Miyajima, F.

AU - Roberts, P.

AU - Pirmohamed, M.

AU - Songer, J. G.

AU - Weese, J. S.

AU - Indra, A.

AU - Corver, J.

AU - Rupnik, M.

AU - Wren, B. W.

AU - Riley, T. V.

AU - Kuijper, E. J.

AU - Lawley, T. D.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The emergence of Clostridium difficile as a significant human diarrheal pathogen is associated with the production of highly transmissible spores and the acquisition of antimicrobial resistance genes (ARGs) and virulence factors. Unlike the hospital-associated C. difficile RT027 lineage, the community-associated C. difficile RT078 lineage is isolated from both humans and farm animals; however, the geographical population structure and transmission networks remain unknown. Here, we applied whole-genome phylogenetic analysis of 248 C. difficile RT078 strains from 22 countries. Our results demonstrate limited geographical clustering for C. difficile RT078 and extensive coclustering of human and animal strains, thereby revealing a highly linked intercontinental transmission network between humans and animals. Comparative whole-genome analysis reveals indistinguishable accessory genomes between human and animal strains and a variety of antimicrobial resistance genes in the pangenome of C. difficile RT078. Thus, bidirectional spread of C. difficile RT078 between farm animals and humans may represent an unappreciated route disseminating antimicrobial resistance genes between humans and animals. These results highlight the importance of the “One Health” concept to monitor infectious disease emergence and the dissemination of antimicrobial resistance genes.

AB - The emergence of Clostridium difficile as a significant human diarrheal pathogen is associated with the production of highly transmissible spores and the acquisition of antimicrobial resistance genes (ARGs) and virulence factors. Unlike the hospital-associated C. difficile RT027 lineage, the community-associated C. difficile RT078 lineage is isolated from both humans and farm animals; however, the geographical population structure and transmission networks remain unknown. Here, we applied whole-genome phylogenetic analysis of 248 C. difficile RT078 strains from 22 countries. Our results demonstrate limited geographical clustering for C. difficile RT078 and extensive coclustering of human and animal strains, thereby revealing a highly linked intercontinental transmission network between humans and animals. Comparative whole-genome analysis reveals indistinguishable accessory genomes between human and animal strains and a variety of antimicrobial resistance genes in the pangenome of C. difficile RT078. Thus, bidirectional spread of C. difficile RT078 between farm animals and humans may represent an unappreciated route disseminating antimicrobial resistance genes between humans and animals. These results highlight the importance of the “One Health” concept to monitor infectious disease emergence and the dissemination of antimicrobial resistance genes.

KW - Accessory genome

KW - Antibiotic resistance

KW - Clostridium difficile

KW - Intercontinental transmission

KW - Interhost transmission

KW - One health concept

KW - RT078

UR - http://www.scopus.com/inward/record.url?scp=85042619817&partnerID=8YFLogxK

U2 - 10.1128/JCM.01384-17

DO - 10.1128/JCM.01384-17

M3 - Article

VL - 56

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

M1 - e01384-17

ER -