Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid

Daniel Fraher, Andrew Sanigorski, Natalie A. Mellett, Peter J. Meikle, Andrew J. Sinclair, Yann Gibert

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)

Abstract

The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.

Original languageEnglish
Pages (from-to)1317-1329
Number of pages13
JournalCell Reports
Volume14
Issue number6
DOIs
Publication statusPublished - 16 Feb 2016
Externally publishedYes

Keywords

  • BODIPY
  • Embryo body
  • Embryogenesis
  • Lipidomics
  • Lipids
  • PPARγ
  • TLC
  • Yolk sac
  • Zebrafish

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