Yersinia enterocoliticaaroA mutants as carriers of the B subunit of the Escherichia coli heat-labile enterotoxin to the murine immune system

Peadar O'Gaora, Mark Roberts, Frances Bowe, Carlos Hormaeche, Raquel Demarco de Hormaeche, Mary Cafferkey, John Tite, Gordon Dougan

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Abstract

Plasmid p5F which directs the expression of the Escherichia coli heat-labile enterotoxin B subunit (LT-B) from the ptac promoter was introduced into the attenuated Yersinia enterocolitica O:8 aroA mutant strain YAM.1. YAM.1 (p5F) expressed high levels of cell-associated and secreted LT-B in a stable fashion when grown on normal laboratory medium. The strain was used as a live oral vaccine in BALB/c mice and vaccinated mice developed high levels of gut-associated and systemic antibodies to both LT-B and the lipopolysaccharide (LPS) of the vaccine strain. Anti-LT-B and anti-LPS responses in the sera were predominantly of the IgG class whereas gut-associated antibodies were predominantly IgA. ELISPOT assays carried out on selected tissues prepared from vaccinated mice showed significant numbers of cells synthesising IgG and IgA antibodies to LT-B. These results show that Y. enterocoliticaaroA mutants can be used effectively as carriers of heterologous antigens to the murine immune system.

Original languageEnglish
Pages (from-to)105-116
Number of pages12
JournalMicrobial Pathogenesis
Volume9
Issue number2
DOIs
Publication statusPublished - 1 Jan 1990
Externally publishedYes

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