Xenon derivatization of halide-soaked protein crystals

Santosh Panjikar, Paul A. Tucker

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.

Original languageEnglish
Pages (from-to)1413-1420
Number of pages8
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number9
DOIs
Publication statusPublished - Sep 2002
Externally publishedYes

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