X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age

Alexandra Grubman, Philipp Guennel, Kirstan A. Vessey, Michael W.M. Jones, Simon A. James, Martin D. De Jonge, Anthony R White, Erica Lucy Fletcher

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The biologically important metals such as zinc, copper and iron play key roles in retinal function, yet no study has mapped the spatio-temporal distribution of retinal biometals in healthy or diseased retina. We investigated a natural mouse model of retinal degeneration, the Cln6nclf mouse. As dysfunctional metabolism of biometals is observed in the brains of these animals and deregulated metal homeostasis has been linked to retinal degeneration, we focused on mapping the elemental distribution in the healthy and Cln6nclf mouse retina with age. Retinal and RPE elemental homeostasis was mapped in Cln6nclf and C57BL6/J mice from 1 to 8 months of age using X-ray Fluorescence Microscopy at the Australian Synchrotron. In the healthy retina, we detected a progressive loss of phosphorus in the outer nuclear layer and significant reduction in iron in the inner segments of the photoreceptors. Further investigation revealed a unique elemental signature for each retinal layer, with high areal concentrations of iron and sulfur in the photoreceptor segments and calcium, phosphorus, zinc and potassium enrichment predominantly in the nuclear layers. The analysis of retinae from Cln6nclf mice did not show significant temporal changes in elemental distributions compared to age matched controls, despite significant photoreceptor cell loss. Our data therefore demonstrates that retinal layers have unique elemental composition. Elemental distribution is, with few exceptions, stably maintained over time in healthy and Cln6nclf mouse retina, suggesting conservation of elemental distribution is critical for basic retinal function with age and is not modulated by processes underlying retinal degeneration.

Original languageEnglish
Pages (from-to)1110-1121
Number of pages12
JournalMetallomics
Volume8
Issue number10
DOIs
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Cite this

Grubman, A., Guennel, P., Vessey, K. A., Jones, M. W. M., James, S. A., De Jonge, M. D., ... Fletcher, E. L. (2016). X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age. Metallomics, 8(10), 1110-1121. https://doi.org/10.1039/c6mt00055j
Grubman, Alexandra ; Guennel, Philipp ; Vessey, Kirstan A. ; Jones, Michael W.M. ; James, Simon A. ; De Jonge, Martin D. ; White, Anthony R ; Fletcher, Erica Lucy. / X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age. In: Metallomics. 2016 ; Vol. 8, No. 10. pp. 1110-1121.
@article{129434429d60425ba71c7bc44acd27da,
title = "X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age",
abstract = "The biologically important metals such as zinc, copper and iron play key roles in retinal function, yet no study has mapped the spatio-temporal distribution of retinal biometals in healthy or diseased retina. We investigated a natural mouse model of retinal degeneration, the Cln6nclf mouse. As dysfunctional metabolism of biometals is observed in the brains of these animals and deregulated metal homeostasis has been linked to retinal degeneration, we focused on mapping the elemental distribution in the healthy and Cln6nclf mouse retina with age. Retinal and RPE elemental homeostasis was mapped in Cln6nclf and C57BL6/J mice from 1 to 8 months of age using X-ray Fluorescence Microscopy at the Australian Synchrotron. In the healthy retina, we detected a progressive loss of phosphorus in the outer nuclear layer and significant reduction in iron in the inner segments of the photoreceptors. Further investigation revealed a unique elemental signature for each retinal layer, with high areal concentrations of iron and sulfur in the photoreceptor segments and calcium, phosphorus, zinc and potassium enrichment predominantly in the nuclear layers. The analysis of retinae from Cln6nclf mice did not show significant temporal changes in elemental distributions compared to age matched controls, despite significant photoreceptor cell loss. Our data therefore demonstrates that retinal layers have unique elemental composition. Elemental distribution is, with few exceptions, stably maintained over time in healthy and Cln6nclf mouse retina, suggesting conservation of elemental distribution is critical for basic retinal function with age and is not modulated by processes underlying retinal degeneration.",
author = "Alexandra Grubman and Philipp Guennel and Vessey, {Kirstan A.} and Jones, {Michael W.M.} and James, {Simon A.} and {De Jonge}, {Martin D.} and White, {Anthony R} and Fletcher, {Erica Lucy}",
year = "2016",
month = "10",
day = "1",
doi = "10.1039/c6mt00055j",
language = "English",
volume = "8",
pages = "1110--1121",
journal = "Metallomics",
issn = "1756-5901",
publisher = "The Royal Society of Chemistry",
number = "10",

}

Grubman, A, Guennel, P, Vessey, KA, Jones, MWM, James, SA, De Jonge, MD, White, AR & Fletcher, EL 2016, 'X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age' Metallomics, vol. 8, no. 10, pp. 1110-1121. https://doi.org/10.1039/c6mt00055j

X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age. / Grubman, Alexandra; Guennel, Philipp; Vessey, Kirstan A.; Jones, Michael W.M.; James, Simon A.; De Jonge, Martin D.; White, Anthony R; Fletcher, Erica Lucy.

In: Metallomics, Vol. 8, No. 10, 01.10.2016, p. 1110-1121.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - X-ray fluorescence microscopic measurement of elemental distribution in the mouse retina with age

AU - Grubman, Alexandra

AU - Guennel, Philipp

AU - Vessey, Kirstan A.

AU - Jones, Michael W.M.

AU - James, Simon A.

AU - De Jonge, Martin D.

AU - White, Anthony R

AU - Fletcher, Erica Lucy

PY - 2016/10/1

Y1 - 2016/10/1

N2 - The biologically important metals such as zinc, copper and iron play key roles in retinal function, yet no study has mapped the spatio-temporal distribution of retinal biometals in healthy or diseased retina. We investigated a natural mouse model of retinal degeneration, the Cln6nclf mouse. As dysfunctional metabolism of biometals is observed in the brains of these animals and deregulated metal homeostasis has been linked to retinal degeneration, we focused on mapping the elemental distribution in the healthy and Cln6nclf mouse retina with age. Retinal and RPE elemental homeostasis was mapped in Cln6nclf and C57BL6/J mice from 1 to 8 months of age using X-ray Fluorescence Microscopy at the Australian Synchrotron. In the healthy retina, we detected a progressive loss of phosphorus in the outer nuclear layer and significant reduction in iron in the inner segments of the photoreceptors. Further investigation revealed a unique elemental signature for each retinal layer, with high areal concentrations of iron and sulfur in the photoreceptor segments and calcium, phosphorus, zinc and potassium enrichment predominantly in the nuclear layers. The analysis of retinae from Cln6nclf mice did not show significant temporal changes in elemental distributions compared to age matched controls, despite significant photoreceptor cell loss. Our data therefore demonstrates that retinal layers have unique elemental composition. Elemental distribution is, with few exceptions, stably maintained over time in healthy and Cln6nclf mouse retina, suggesting conservation of elemental distribution is critical for basic retinal function with age and is not modulated by processes underlying retinal degeneration.

AB - The biologically important metals such as zinc, copper and iron play key roles in retinal function, yet no study has mapped the spatio-temporal distribution of retinal biometals in healthy or diseased retina. We investigated a natural mouse model of retinal degeneration, the Cln6nclf mouse. As dysfunctional metabolism of biometals is observed in the brains of these animals and deregulated metal homeostasis has been linked to retinal degeneration, we focused on mapping the elemental distribution in the healthy and Cln6nclf mouse retina with age. Retinal and RPE elemental homeostasis was mapped in Cln6nclf and C57BL6/J mice from 1 to 8 months of age using X-ray Fluorescence Microscopy at the Australian Synchrotron. In the healthy retina, we detected a progressive loss of phosphorus in the outer nuclear layer and significant reduction in iron in the inner segments of the photoreceptors. Further investigation revealed a unique elemental signature for each retinal layer, with high areal concentrations of iron and sulfur in the photoreceptor segments and calcium, phosphorus, zinc and potassium enrichment predominantly in the nuclear layers. The analysis of retinae from Cln6nclf mice did not show significant temporal changes in elemental distributions compared to age matched controls, despite significant photoreceptor cell loss. Our data therefore demonstrates that retinal layers have unique elemental composition. Elemental distribution is, with few exceptions, stably maintained over time in healthy and Cln6nclf mouse retina, suggesting conservation of elemental distribution is critical for basic retinal function with age and is not modulated by processes underlying retinal degeneration.

UR - http://www.scopus.com/inward/record.url?scp=84991497756&partnerID=8YFLogxK

U2 - 10.1039/c6mt00055j

DO - 10.1039/c6mt00055j

M3 - Article

VL - 8

SP - 1110

EP - 1121

JO - Metallomics

JF - Metallomics

SN - 1756-5901

IS - 10

ER -