@article{a7014d1f3a914ba19a087e83a9b9f86e,
title = "Wounding and methyl jasmonate increase cyanogenic glucoside concentrations in Sorghum bicolor via upregulation of biosynthesis",
abstract = "The cyanogenic glucoside, dhurrin, present in Sorghum bicolor is thought to have multiple functions, including in defence against herbivory. The hormone methyl jasmonate (MeJA) is also induced by herbivory and is key to instigating defence processes in plants. To investigate whether dhurrin is induced in response to herbivore attack and also to the associated presence of MeJA, sorghum plants were either wounded or exogenous MeJA was applied. We show that specific wounding (pin board and perforation) and the application of MeJA increases dhurrin concentration in leaves and sheath tissue 12 h after treatment. Quantitative PCR shows that the expression of two genes, SbCYP79A1 and SbUGT85B1, involved in the synthesis of dhurrin are significantly induced by exogenous MeJA and by wounding. Analysis of 2 kb of sequence upstream of the start codon of SbCYP79A1 identifies several cis-acting elements that have been linked to MeJA induction. A promoter deletion series, coupled to GFP, and transiently expressed in Nicotiana benthamiana suggests that there are potentially three sequence motifs (~−925 to −976) involved in the binding of transcription factors that result in increased expression of SbCYP79A1 and the synthesis of dhurrin in response to MeJA.",
keywords = "Cyanogenesis, Dhurrin, gene expression, herbivory, MeJA, plant defence, wounding",
author = "Sohail, {M. N.} and O'Donnell, {N. H.} and Kaiser, {B. N.} and Blomstedt, {C. K.} and Gleadow, {R. M.}",
note = "Funding Information: MNS was supported by Monash Graduate Scholarship and Monash International Postgraduate Research Scholarship. NO'D was supported by a Dean's PhD Scholarship from the School of Biological Sciences, Monash University. This project was supported by an Australian Research Council Linkage Grant with Pacific Seeds Pty. Ltd (LP100100434) and an ARC Discovery Project grant (DP130101049) to RMG. Open access publishing facilitated by Monash University, as part of the Wiley ‐ Monash University agreement via the Council of Australian University Librarians. Funding Information: MN Sohail, RM Gleadow and CK Blomstedt designed the methyl jasmonate experiments and analysed the results. NH O{\textquoteright}Donnell, RM Gleadow and CK Blomstedt designed the wounding experiments, in collaboration with AD Neale and JD Hamill, Monash University. MN Sohail and CK Blomstedt carried out the gene expression studies. RM Gleadow was the overall project supervisor. All authors contributed to writing and editing of the manuscript. MN Sohail was supported by a Monash Graduate Scholarship and a Monash International Postgraduate Research Scholarship. NH O{\textquoteright}Donnell was supported by a Dean's PhD Scholarship from the School of Biological Sciences, Monash University. The project was supported by Australian Research Council Grants through RM Gleadow with an ARC Linkage Grant with Pacific Seeds Pty. Ltd (LP100100434) and an ARC Discovery Project grant (DP130101049). Open access publishing facilitated by Monash University, as part of the Wiley ‐ Monash University agreement via the Council of Australian University Librarians. Funding Information: MNS was supported by Monash Graduate Scholarship and Monash International Postgraduate Research Scholarship. NO'D was supported by a Dean's PhD Scholarship from the School of Biological Sciences, Monash University. This project was supported by an Australian Research Council Linkage Grant with Pacific Seeds Pty. Ltd (LP100100434) and an ARC Discovery Project grant (DP130101049) to RMG. Open access publishing facilitated by Monash University, as part of the Wiley - Monash University agreement via the Council of Australian University Librarians. Publisher Copyright: {\textcopyright} 2023 The Authors. Plant Biology published by John Wiley & Sons Ltd on behalf of German Society for Plant Sciences, Royal Botanical Society of the Netherlands.",
year = "2023",
month = jun,
doi = "10.1111/plb.13522",
language = "English",
volume = "25",
pages = "498--508",
journal = "Plant Biology",
issn = "1435-8603",
publisher = "Wiley-Blackwell",
number = "4",
}