Wolbachia infections of phlebotomine sand flies (Diptera: Psychodidae)

Midori Ono, Henk Braig, Leonard Munstermann, Cristina Ferro, Scott Leslie O'Neill

Research output: Contribution to journalArticleResearchpeer-review

43 Citations (Scopus)

Abstract

Old and New World phlebotomine sand fly species were screened for infection with Wolbachia, intracellular bacterial endosymbionts found in many arthropods and filarial nematodes. Of 53 samples representing 15 species, nine samples of four species were found positive for Wolbachia by polymerase chain reaction amplification using primers for the Wolbachia surface protein ((wsp) gene. Five of the wsi, gene fragments from four species were cloned, sequenced, and used for phylogenetic analysis. These wsp sequences were placed in three different clades within the arthropod associated Wolbachia (groups A and B), suggesting that Wolbachia has infected sand flies on more than one occasion. Two distantly related sand fly species, Lutzomyia (Psanthromyia) shannoni (Dyar) and Lutzomyia (Nyssomyia) whitmani (Antunes Coutinho), infected with an identical Wolbachia strain suggest a very recent horizontal transmission.
Original languageEnglish
Pages (from-to)237 - 241
Number of pages5
JournalJournal of Medical Entomology
Volume38
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

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