Wnt regulation of chondrocyte differentiation

Vicki Church, Tsutomu Nohno, Christophe Marcelle, Claudia Linker, Philippa Francis-West

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The Wnt family of growth factors are important regulators of several developmental processes including skeletogenesis. To further investigate the role of Wnts we analysed their expression in the developing chick limb and performed functional analyses in vivo and in vitro. We found that Wnt5b and Wnt11 are restricted within the prehypertrophic chondrocytes of the cartilage elements, Wnt5a is found in the joints and perichondrium, while Wnt4 is expressed in the developing joints and, in some bones, a subset of the hypertrophic chondrocytes. These Wnts mediate distinct effects on the initiation of chondrogenesis and differentiation of chondrocytes in vitro and in vivo. Wnt4 blocks the initiation of chondrogenesis and accelerates terminal chondrocyte differentiation in vitro. In contrast, Wnt5a and Wnt5b promote early chondrogenesis in vitro while inhibiting terminal differentiation in vivo. As Wnt5b and Wnt11 expression overlaps with and appears after Indian hedgehog (Ihh), we also compared their effects with Ihh to see if they mediate aspects of Ihh signalling. This showed that Ihh and Wnt5b and Wnt11 control chondrogenesis in parallel pathways.
Original languageEnglish
Pages (from-to)4809 - 4818
Number of pages10
JournalJournal of Cell Science
Volume115
Issue numberPt. 2
Publication statusPublished - 2002

Cite this

Church, V., Nohno, T., Marcelle, C., Linker, C., & Francis-West, P. (2002). Wnt regulation of chondrocyte differentiation. Journal of Cell Science, 115(Pt. 2), 4809 - 4818.
Church, Vicki ; Nohno, Tsutomu ; Marcelle, Christophe ; Linker, Claudia ; Francis-West, Philippa. / Wnt regulation of chondrocyte differentiation. In: Journal of Cell Science. 2002 ; Vol. 115, No. Pt. 2. pp. 4809 - 4818.
@article{194bb5245d8b40eda22dee4676a1827a,
title = "Wnt regulation of chondrocyte differentiation",
abstract = "The Wnt family of growth factors are important regulators of several developmental processes including skeletogenesis. To further investigate the role of Wnts we analysed their expression in the developing chick limb and performed functional analyses in vivo and in vitro. We found that Wnt5b and Wnt11 are restricted within the prehypertrophic chondrocytes of the cartilage elements, Wnt5a is found in the joints and perichondrium, while Wnt4 is expressed in the developing joints and, in some bones, a subset of the hypertrophic chondrocytes. These Wnts mediate distinct effects on the initiation of chondrogenesis and differentiation of chondrocytes in vitro and in vivo. Wnt4 blocks the initiation of chondrogenesis and accelerates terminal chondrocyte differentiation in vitro. In contrast, Wnt5a and Wnt5b promote early chondrogenesis in vitro while inhibiting terminal differentiation in vivo. As Wnt5b and Wnt11 expression overlaps with and appears after Indian hedgehog (Ihh), we also compared their effects with Ihh to see if they mediate aspects of Ihh signalling. This showed that Ihh and Wnt5b and Wnt11 control chondrogenesis in parallel pathways.",
author = "Vicki Church and Tsutomu Nohno and Christophe Marcelle and Claudia Linker and Philippa Francis-West",
year = "2002",
language = "English",
volume = "115",
pages = "4809 -- 4818",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists",
number = "Pt. 2",

}

Church, V, Nohno, T, Marcelle, C, Linker, C & Francis-West, P 2002, 'Wnt regulation of chondrocyte differentiation' Journal of Cell Science, vol. 115, no. Pt. 2, pp. 4809 - 4818.

Wnt regulation of chondrocyte differentiation. / Church, Vicki; Nohno, Tsutomu; Marcelle, Christophe; Linker, Claudia; Francis-West, Philippa.

In: Journal of Cell Science, Vol. 115, No. Pt. 2, 2002, p. 4809 - 4818.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Wnt regulation of chondrocyte differentiation

AU - Church, Vicki

AU - Nohno, Tsutomu

AU - Marcelle, Christophe

AU - Linker, Claudia

AU - Francis-West, Philippa

PY - 2002

Y1 - 2002

N2 - The Wnt family of growth factors are important regulators of several developmental processes including skeletogenesis. To further investigate the role of Wnts we analysed their expression in the developing chick limb and performed functional analyses in vivo and in vitro. We found that Wnt5b and Wnt11 are restricted within the prehypertrophic chondrocytes of the cartilage elements, Wnt5a is found in the joints and perichondrium, while Wnt4 is expressed in the developing joints and, in some bones, a subset of the hypertrophic chondrocytes. These Wnts mediate distinct effects on the initiation of chondrogenesis and differentiation of chondrocytes in vitro and in vivo. Wnt4 blocks the initiation of chondrogenesis and accelerates terminal chondrocyte differentiation in vitro. In contrast, Wnt5a and Wnt5b promote early chondrogenesis in vitro while inhibiting terminal differentiation in vivo. As Wnt5b and Wnt11 expression overlaps with and appears after Indian hedgehog (Ihh), we also compared their effects with Ihh to see if they mediate aspects of Ihh signalling. This showed that Ihh and Wnt5b and Wnt11 control chondrogenesis in parallel pathways.

AB - The Wnt family of growth factors are important regulators of several developmental processes including skeletogenesis. To further investigate the role of Wnts we analysed their expression in the developing chick limb and performed functional analyses in vivo and in vitro. We found that Wnt5b and Wnt11 are restricted within the prehypertrophic chondrocytes of the cartilage elements, Wnt5a is found in the joints and perichondrium, while Wnt4 is expressed in the developing joints and, in some bones, a subset of the hypertrophic chondrocytes. These Wnts mediate distinct effects on the initiation of chondrogenesis and differentiation of chondrocytes in vitro and in vivo. Wnt4 blocks the initiation of chondrogenesis and accelerates terminal chondrocyte differentiation in vitro. In contrast, Wnt5a and Wnt5b promote early chondrogenesis in vitro while inhibiting terminal differentiation in vivo. As Wnt5b and Wnt11 expression overlaps with and appears after Indian hedgehog (Ihh), we also compared their effects with Ihh to see if they mediate aspects of Ihh signalling. This showed that Ihh and Wnt5b and Wnt11 control chondrogenesis in parallel pathways.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12432069

M3 - Article

VL - 115

SP - 4809

EP - 4818

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - Pt. 2

ER -

Church V, Nohno T, Marcelle C, Linker C, Francis-West P. Wnt regulation of chondrocyte differentiation. Journal of Cell Science. 2002;115(Pt. 2):4809 - 4818.