WAFL, a new protein involved in regulation of early endocytic transport at the intersection of actin and microtubule dynamics

Ing Marie Viklund, Pontus Aspenström, Vannary Meas-Yedid, Bo Zhang, Jolanta Kopec, Daniel Ågren, Gunter Schneider, Mauro D'Amato, Jean Christophe Olivo-Marin, Philippe Sansonetti, Guy Tran Van Nhieu, Sven Pettersson

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20 Citations (Scopus)

Abstract

We have previously identified a new gene with sequence homology to the WASP-family of actin regulators denoted WAFL (WASP and FKBP-like). Here we report a possible biological function for WAFL, by demonstrating an association to early endosomes via its central coiled-coil domain. Further we show by functional and structural studies that WAFL is associated with both microtubules and the actin filament system, the two means of transport of early endosomes. In addition, WAFL interacts with WASP-interacting protein (WIP) and actin, thus linking WAFL to actin dynamics. The use of RNAi depletion of WAFL shows that WAFL-deficient cells display delayed transport of endosomal cargo. Our findings are compatible with a model whereby WAFL is involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.

Original languageEnglish
Pages (from-to)1040-1052
Number of pages13
JournalExperimental Cell Research
Volume315
Issue number6
DOIs
Publication statusPublished - 1 Apr 2009
Externally publishedYes

Keywords

  • Actin
  • Endocytosis
  • FKBP133
  • FKBP15
  • KIAA0674
  • Microtubules
  • Transport
  • WIP

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