Vsx2 in the zebrafish retina: Restricted lineages through derepression

Marta Vitorino, Patricia Regina Jusuf, Daniel Maurus, Yukiko Kimura, Shinichi Higashijima, William Harris

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Abstract

BACKGROUND: The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent. RESULTS: In this study we show that the homeodomain transcription factor Vsx2 is initially expressed throughout the retinal epithelium, but later it is downregulated in all but a minor population of bipolar cells and all Muller glia. The Vsx2-negative daughters of Vsx2-positive RPCs divide and give rise to all other cell types in the retina. Vsx2 is a repressor whose targets include transcription factors such as Vsx1, which is expressed in the progenitors of distinct non-Vsx2 bipolars, and the basic helix-loop-helix transcription factor Ath5, which restricts the fate of progenitors to retinal ganglion cells, horizontal cells, amacrine cells and photoreceptors fates. Foxn4, expressed in the progenitors of amacrine and horizontal cells, is also negatively regulated by Vsx2. CONCLUSION: Our data thus suggest Vsx2-positive RPCs are fully multipotent retinal progenitors and that when Vsx2 is downregulated, Vsx2-negative progenitors escape Vsx2 repression and so are able to express factors that restrict lineage potential.
Original languageEnglish
Pages (from-to)1 - 26
Number of pages26
JournalNeural Development
Volume4
Issue numberArt. No: 14
DOIs
Publication statusPublished - 2009

Cite this

Vitorino, M., Jusuf, P. R., Maurus, D., Kimura, Y., Higashijima, S., & Harris, W. (2009). Vsx2 in the zebrafish retina: Restricted lineages through derepression. Neural Development, 4(Art. No: 14), 1 - 26. https://doi.org/10.1186/1749-8104-4-14
Vitorino, Marta ; Jusuf, Patricia Regina ; Maurus, Daniel ; Kimura, Yukiko ; Higashijima, Shinichi ; Harris, William. / Vsx2 in the zebrafish retina: Restricted lineages through derepression. In: Neural Development. 2009 ; Vol. 4, No. Art. No: 14. pp. 1 - 26.
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title = "Vsx2 in the zebrafish retina: Restricted lineages through derepression",
abstract = "BACKGROUND: The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent. RESULTS: In this study we show that the homeodomain transcription factor Vsx2 is initially expressed throughout the retinal epithelium, but later it is downregulated in all but a minor population of bipolar cells and all Muller glia. The Vsx2-negative daughters of Vsx2-positive RPCs divide and give rise to all other cell types in the retina. Vsx2 is a repressor whose targets include transcription factors such as Vsx1, which is expressed in the progenitors of distinct non-Vsx2 bipolars, and the basic helix-loop-helix transcription factor Ath5, which restricts the fate of progenitors to retinal ganglion cells, horizontal cells, amacrine cells and photoreceptors fates. Foxn4, expressed in the progenitors of amacrine and horizontal cells, is also negatively regulated by Vsx2. CONCLUSION: Our data thus suggest Vsx2-positive RPCs are fully multipotent retinal progenitors and that when Vsx2 is downregulated, Vsx2-negative progenitors escape Vsx2 repression and so are able to express factors that restrict lineage potential.",
author = "Marta Vitorino and Jusuf, {Patricia Regina} and Daniel Maurus and Yukiko Kimura and Shinichi Higashijima and William Harris",
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Vitorino, M, Jusuf, PR, Maurus, D, Kimura, Y, Higashijima, S & Harris, W 2009, 'Vsx2 in the zebrafish retina: Restricted lineages through derepression', Neural Development, vol. 4, no. Art. No: 14, pp. 1 - 26. https://doi.org/10.1186/1749-8104-4-14

Vsx2 in the zebrafish retina: Restricted lineages through derepression. / Vitorino, Marta; Jusuf, Patricia Regina; Maurus, Daniel; Kimura, Yukiko; Higashijima, Shinichi; Harris, William.

In: Neural Development, Vol. 4, No. Art. No: 14, 2009, p. 1 - 26.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Vsx2 in the zebrafish retina: Restricted lineages through derepression

AU - Vitorino, Marta

AU - Jusuf, Patricia Regina

AU - Maurus, Daniel

AU - Kimura, Yukiko

AU - Higashijima, Shinichi

AU - Harris, William

PY - 2009

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N2 - BACKGROUND: The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent. RESULTS: In this study we show that the homeodomain transcription factor Vsx2 is initially expressed throughout the retinal epithelium, but later it is downregulated in all but a minor population of bipolar cells and all Muller glia. The Vsx2-negative daughters of Vsx2-positive RPCs divide and give rise to all other cell types in the retina. Vsx2 is a repressor whose targets include transcription factors such as Vsx1, which is expressed in the progenitors of distinct non-Vsx2 bipolars, and the basic helix-loop-helix transcription factor Ath5, which restricts the fate of progenitors to retinal ganglion cells, horizontal cells, amacrine cells and photoreceptors fates. Foxn4, expressed in the progenitors of amacrine and horizontal cells, is also negatively regulated by Vsx2. CONCLUSION: Our data thus suggest Vsx2-positive RPCs are fully multipotent retinal progenitors and that when Vsx2 is downregulated, Vsx2-negative progenitors escape Vsx2 repression and so are able to express factors that restrict lineage potential.

AB - BACKGROUND: The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent. RESULTS: In this study we show that the homeodomain transcription factor Vsx2 is initially expressed throughout the retinal epithelium, but later it is downregulated in all but a minor population of bipolar cells and all Muller glia. The Vsx2-negative daughters of Vsx2-positive RPCs divide and give rise to all other cell types in the retina. Vsx2 is a repressor whose targets include transcription factors such as Vsx1, which is expressed in the progenitors of distinct non-Vsx2 bipolars, and the basic helix-loop-helix transcription factor Ath5, which restricts the fate of progenitors to retinal ganglion cells, horizontal cells, amacrine cells and photoreceptors fates. Foxn4, expressed in the progenitors of amacrine and horizontal cells, is also negatively regulated by Vsx2. CONCLUSION: Our data thus suggest Vsx2-positive RPCs are fully multipotent retinal progenitors and that when Vsx2 is downregulated, Vsx2-negative progenitors escape Vsx2 repression and so are able to express factors that restrict lineage potential.

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