Volume Microscopy of Nudivirus Infected Cells

Bruno M. Humbel; Sailakshmi Velamoor; Allan Mitchell; Mihnea Bostina

doi:10.1007/978-981-16-2982-2_25

Volume Microscopy of Nudivirus Infected Cells

Bruno M. Humbel, Sailakshmi Velamoor, Allan Mitchell, Mihnea Bostina

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

1 Citation (Scopus)

Abstract

Volume microscopy is an ideal method to investigate microbial infections. In this study, we used Oryctes rhinoceros nudivirus (OrNV) as a model to illustrate the power of this technology in deciphering morphological changes associated with viral replication. Nudiviruses are large dsDNA rod-shaped enveloped viruses infecting a wide range of hosts. The best characterized member of the family, OrNV, infects rhinoceros beetle, a devastating pest damaging coconut and oil palm trees in Southeast Asia and the Pacific islands. Although previous electron microscopy studies have described the cellular changes associated with OrNV infection, little is known regarding the mechanism of viral assembly and egress. Here we used focussed ion beam and scanning electron microscopy to characterize the cellular remodelling associated with OrNV infection.

Original language	English
Title of host publication	Applications of Microscopy in Materials and Life Sciences
Subtitle of host publication	Proceedings of 12th Asia-Pacific Microscopy Conference
Editors	Partha Ghosal, C. Barry Carter, Kutti Ragunath Vinothkumar, Rajdeep Sarkar
Place of Publication	Singapore
Publisher	Springer
Pages	251-259
Number of pages	9
ISBN (Electronic)	9789811629822
ISBN (Print)	9789811629815
DOIs	https://doi.org/10.1007/978-981-16-2982-2_25
Publication status	Published - 2021
Externally published	Yes
Event	Asia-Pacific Microscopy Conference 2020 - Hyderabad, India Duration: 3 Feb 2020 → 7 Feb 2020 Conference number: 12th https://link.springer.com/book/10.1007/978-981-16-2982-2

Publication series

Name	Springer Proceedings in Materials
Volume	11
ISSN (Print)	2662-3161
ISSN (Electronic)	2662-317X

Conference

Conference	Asia-Pacific Microscopy Conference 2020
Abbreviated title	APMC12
Country/Territory	India
City	Hyderabad
Period	3/02/20 → 7/02/20
Internet address	https://link.springer.com/book/10.1007/978-981-16-2982-2

Keywords

FIB-SEM
Nudiviruses
Virus assembly
Virus egress
Volume microscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-981-16-2982-2_25

Cite this

Humbel, B. M., Velamoor, S., Mitchell, A., & Bostina, M. (2021). Volume Microscopy of Nudivirus Infected Cells. In P. Ghosal, C. B. Carter, K. R. Vinothkumar, & R. Sarkar (Eds.), Applications of Microscopy in Materials and Life Sciences: Proceedings of 12th Asia-Pacific Microscopy Conference (pp. 251-259). (Springer Proceedings in Materials; Vol. 11). Springer. https://doi.org/10.1007/978-981-16-2982-2_25

Humbel, Bruno M. ; Velamoor, Sailakshmi ; Mitchell, Allan et al. / Volume Microscopy of Nudivirus Infected Cells. Applications of Microscopy in Materials and Life Sciences: Proceedings of 12th Asia-Pacific Microscopy Conference. editor / Partha Ghosal ; C. Barry Carter ; Kutti Ragunath Vinothkumar ; Rajdeep Sarkar. Singapore : Springer, 2021. pp. 251-259 (Springer Proceedings in Materials).

@inproceedings{8f9988f443274bde9078a73644c9c20e,

title = "Volume Microscopy of Nudivirus Infected Cells",

abstract = "Volume microscopy is an ideal method to investigate microbial infections. In this study, we used Oryctes rhinoceros nudivirus (OrNV) as a model to illustrate the power of this technology in deciphering morphological changes associated with viral replication. Nudiviruses are large dsDNA rod-shaped enveloped viruses infecting a wide range of hosts. The best characterized member of the family, OrNV, infects rhinoceros beetle, a devastating pest damaging coconut and oil palm trees in Southeast Asia and the Pacific islands. Although previous electron microscopy studies have described the cellular changes associated with OrNV infection, little is known regarding the mechanism of viral assembly and egress. Here we used focussed ion beam and scanning electron microscopy to characterize the cellular remodelling associated with OrNV infection.",

keywords = "FIB-SEM, Nudiviruses, Virus assembly, Virus egress, Volume microscopy",

author = "Humbel, {Bruno M.} and Sailakshmi Velamoor and Allan Mitchell and Mihnea Bostina",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.; Asia-Pacific Microscopy Conference 2020, APMC12 ; Conference date: 03-02-2020 Through 07-02-2020",

year = "2021",

doi = "10.1007/978-981-16-2982-2_25",

language = "English",

isbn = "9789811629815",

series = "Springer Proceedings in Materials",

publisher = "Springer",

pages = "251--259",

editor = "Partha Ghosal and Carter, {C. Barry} and Vinothkumar, {Kutti Ragunath} and Rajdeep Sarkar",

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Humbel, BM, Velamoor, S, Mitchell, A & Bostina, M 2021, Volume Microscopy of Nudivirus Infected Cells. in P Ghosal, CB Carter, KR Vinothkumar & R Sarkar (eds), Applications of Microscopy in Materials and Life Sciences: Proceedings of 12th Asia-Pacific Microscopy Conference. Springer Proceedings in Materials, vol. 11, Springer, Singapore, pp. 251-259, Asia-Pacific Microscopy Conference 2020, Hyderabad, India, 3/02/20. https://doi.org/10.1007/978-981-16-2982-2_25

Volume Microscopy of Nudivirus Infected Cells. / Humbel, Bruno M.; Velamoor, Sailakshmi; Mitchell, Allan et al.
Applications of Microscopy in Materials and Life Sciences: Proceedings of 12th Asia-Pacific Microscopy Conference. ed. / Partha Ghosal; C. Barry Carter; Kutti Ragunath Vinothkumar; Rajdeep Sarkar. Singapore: Springer, 2021. p. 251-259 (Springer Proceedings in Materials; Vol. 11).

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Volume Microscopy of Nudivirus Infected Cells

AU - Humbel, Bruno M.

AU - Velamoor, Sailakshmi

AU - Mitchell, Allan

AU - Bostina, Mihnea

N1 - Conference code: 12th

PY - 2021

Y1 - 2021

N2 - Volume microscopy is an ideal method to investigate microbial infections. In this study, we used Oryctes rhinoceros nudivirus (OrNV) as a model to illustrate the power of this technology in deciphering morphological changes associated with viral replication. Nudiviruses are large dsDNA rod-shaped enveloped viruses infecting a wide range of hosts. The best characterized member of the family, OrNV, infects rhinoceros beetle, a devastating pest damaging coconut and oil palm trees in Southeast Asia and the Pacific islands. Although previous electron microscopy studies have described the cellular changes associated with OrNV infection, little is known regarding the mechanism of viral assembly and egress. Here we used focussed ion beam and scanning electron microscopy to characterize the cellular remodelling associated with OrNV infection.

AB - Volume microscopy is an ideal method to investigate microbial infections. In this study, we used Oryctes rhinoceros nudivirus (OrNV) as a model to illustrate the power of this technology in deciphering morphological changes associated with viral replication. Nudiviruses are large dsDNA rod-shaped enveloped viruses infecting a wide range of hosts. The best characterized member of the family, OrNV, infects rhinoceros beetle, a devastating pest damaging coconut and oil palm trees in Southeast Asia and the Pacific islands. Although previous electron microscopy studies have described the cellular changes associated with OrNV infection, little is known regarding the mechanism of viral assembly and egress. Here we used focussed ion beam and scanning electron microscopy to characterize the cellular remodelling associated with OrNV infection.

KW - FIB-SEM

KW - Nudiviruses

KW - Virus assembly

KW - Virus egress

KW - Volume microscopy

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U2 - 10.1007/978-981-16-2982-2_25

DO - 10.1007/978-981-16-2982-2_25

M3 - Conference Paper

AN - SCOPUS:85127881959

SN - 9789811629815

T3 - Springer Proceedings in Materials

SP - 251

EP - 259

BT - Applications of Microscopy in Materials and Life Sciences

A2 - Ghosal, Partha

A2 - Carter, C. Barry

A2 - Vinothkumar, Kutti Ragunath

A2 - Sarkar, Rajdeep

PB - Springer

CY - Singapore

T2 - Asia-Pacific Microscopy Conference 2020

Y2 - 3 February 2020 through 7 February 2020

ER -

Humbel BM, Velamoor S, Mitchell A, Bostina M. Volume Microscopy of Nudivirus Infected Cells. In Ghosal P, Carter CB, Vinothkumar KR, Sarkar R, editors, Applications of Microscopy in Materials and Life Sciences: Proceedings of 12th Asia-Pacific Microscopy Conference. Singapore: Springer. 2021. p. 251-259. (Springer Proceedings in Materials). doi: 10.1007/978-981-16-2982-2_25

Volume Microscopy of Nudivirus Infected Cells

Abstract

Publication series

Conference

Keywords

UN SDGs

Access to Document

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Cite this