Volta phase plate cryo-EM of the small protein complex Prx3

Maryam Khoshouei, Mazdak Radjainia, Amy J. Phillips, Juliet A Gerrard, Alok K Mitra, Jürgen M. Plitzko, Wolfgang Baumeister , Radostin Danev

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Abstract

Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.

Original languageEnglish
Article number10534
Number of pages5
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 28 Jan 2016

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