Vitellibacter aquimaris sp. nov., a marine bacterium isolated from seawater

Suganthi Thevarajoo, Chitra Selvaratnam, Kian Mau Goh, Kar Wai Hong, Xin Yue Chan, Kok Gan Chan, Chun Shiong Chong

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14 Citations (Scopus)

Abstract

A Gram-staining-negative, aerobic, yellow–orange-pigmented, rod-shaped bacterium designated D-24T was isolated from seawater from sandy shoreline in Johor, Malaysia. The 16S rRNA gene sequence analysis revealed that strain D-24T is affiliated with the genus Vitellibacter. It shared more than 96% sequence similarity with the types of some of the validly published species of the genus: Vitellibacter vladivostokensis KMM 3516T (99.5 %), Vitellibacter soesokkakensis RSSK- 12T (97.3 %), Vitellibacter echinoideorum CC-CZW007T (96.9 %), Vitellibacter nionensis VBW088T (96.7 %) and Vitellibacter aestuarii JCM 15496T (96.3 %). DNA–DNA hybridization and genome-based analysis of average nucleotide identity (ANI) of strain D-24T versus V. vladivostokensis KMM 3516T exhibited values of 35.9±0.14% and 89.26 %, respectively. Strain D-24T showed an even lower ANI value of 80.88% with V. soesokkakensis RSSK-12T. The major menaquinone of strain D-24T was MK-6, and the predominant fatty acids were iso-C15: 0 and iso-C17: 0 3-OH. Strain D-24T contained major amounts of phosphatidylethanolamine, two lipids and two aminolipids, and a phosphoglycolipid that was different to that of other species of the genus Vitellibacter. The genomic DNA G+C content was 40.6 mol%. On the basis of phenotypic properties, DNA–DNA relatedness, ANI value and chemotaxonomic analyses, strain D-24T represents a novel species of the genus Vitellibacter, for which the name Vitellibacter aquimaris sp. nov. is proposed. The type strain is D-24T (=KCTC 42708T=DSM 101732T).

Original languageEnglish
Article number001248
Pages (from-to)3662-3668
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number9
DOIs
Publication statusPublished - Sept 2016
Externally publishedYes

Keywords

  • Average nucleotide identity
  • DNA-DNA hybridization
  • Flavobacteriaceae
  • Marine bacterium
  • Vitellibacter aquimaris

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