Vitellibacter aquimaris sp. nov., a marine bacterium isolated from seawater

A Gram-staining-negative, aerobic, yellow–orange-pigmented, rod-shaped bacterium designated D-24^T was isolated from seawater from sandy shoreline in Johor, Malaysia. The 16S rRNA gene sequence analysis revealed that strain D-24^T is affiliated with the genus Vitellibacter. It shared more than 96% sequence similarity with the types of some of the validly published species of the genus: Vitellibacter vladivostokensis KMM 3516^T (99.5 %), Vitellibacter soesokkakensis RSSK- 12^T (97.3 %), Vitellibacter echinoideorum CC-CZW007^T (96.9 %), Vitellibacter nionensis VBW088^T (96.7 %) and Vitellibacter aestuarii JCM 15496^T (96.3 %). DNA–DNA hybridization and genome-based analysis of average nucleotide identity (ANI) of strain D-24^T versus V. vladivostokensis KMM 3516^T exhibited values of 35.9±0.14% and 89.26 %, respectively. Strain D-24^T showed an even lower ANI value of 80.88% with V. soesokkakensis RSSK-12^T. The major menaquinone of strain D-24^T was MK-6, and the predominant fatty acids were iso-C_{15: 0} and iso-C_{17: 0} 3-OH. Strain D-24^T contained major amounts of phosphatidylethanolamine, two lipids and two aminolipids, and a phosphoglycolipid that was different to that of other species of the genus Vitellibacter. The genomic DNA G+C content was 40.6 mol%. On the basis of phenotypic properties, DNA–DNA relatedness, ANI value and chemotaxonomic analyses, strain D-24^T represents a novel species of the genus Vitellibacter, for which the name Vitellibacter aquimaris sp. nov. is proposed. The type strain is D-24^T (=KCTC 42708^T=DSM 101732^T).