TY - JOUR
T1 - Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma
AU - Albarhani, Ali A.
AU - Collier, Fiona
AU - Greaves, Ronda F.
AU - Ponsonby, Anne Louise
AU - Allen, Katrina J.
AU - Vuillermin, Peter J.
AU - Roche, Peter
AU - Clarke, Michael W.
AU - BIS Steering Committee
AU - Burgner, David
AU - Carlin, John
AU - Saffery, Richard
PY - 2015/1/16
Y1 - 2015/1/16
N2 - Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14. nmol/L [95% confidence interval (95% CI), - 6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2. nmol/L [95% CI, - 9.5 to 13.9] and 4.1. nmol/L [95% CI, - 14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07. μmol/L [95% CI, - 0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.
AB - Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14. nmol/L [95% confidence interval (95% CI), - 6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2. nmol/L [95% CI, - 9.5 to 13.9] and 4.1. nmol/L [95% CI, - 14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07. μmol/L [95% CI, - 0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.
KW - Cholecalciferol
KW - Mass spectrometry
KW - Neonates
KW - Retinol
KW - Umbilical cord blood diluted plasma
UR - http://www.scopus.com/inward/record.url?scp=84947044419&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2015.04.014
DO - 10.1016/j.clinbiochem.2015.04.014
M3 - Article
C2 - 25920890
AN - SCOPUS:84947044419
SN - 0009-9120
VL - 48
SP - 1105
EP - 1112
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 16-17
ER -