Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

Ali A. Albarhani, Fiona Collier, Ronda F. Greaves, Anne Louise Ponsonby, Katrina J. Allen, Peter J. Vuillermin, Peter Roche, Michael W. Clarke, BIS Steering Committee

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Abstract

Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14. nmol/L [95% confidence interval (95% CI), - 6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2. nmol/L [95% CI, - 9.5 to 13.9] and 4.1. nmol/L [95% CI, - 14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07. μmol/L [95% CI, - 0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.

Original languageEnglish
Pages (from-to)1105-1112
Number of pages8
JournalClinical Biochemistry
Volume48
Issue number16-17
DOIs
Publication statusPublished - 16 Jan 2015

Keywords

  • Cholecalciferol
  • Mass spectrometry
  • Neonates
  • Retinol
  • Umbilical cord blood diluted plasma

Cite this

Albarhani, A. A., Collier, F., Greaves, R. F., Ponsonby, A. L., Allen, K. J., Vuillermin, P. J., ... BIS Steering Committee (2015). Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma. Clinical Biochemistry, 48(16-17), 1105-1112. https://doi.org/10.1016/j.clinbiochem.2015.04.014
Albarhani, Ali A. ; Collier, Fiona ; Greaves, Ronda F. ; Ponsonby, Anne Louise ; Allen, Katrina J. ; Vuillermin, Peter J. ; Roche, Peter ; Clarke, Michael W. ; BIS Steering Committee. / Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma. In: Clinical Biochemistry. 2015 ; Vol. 48, No. 16-17. pp. 1105-1112.
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title = "Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma",
abstract = "Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14. nmol/L [95{\%} confidence interval (95{\%} CI), - 6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2. nmol/L [95{\%} CI, - 9.5 to 13.9] and 4.1. nmol/L [95{\%} CI, - 14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07. μmol/L [95{\%} CI, - 0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.",
keywords = "Cholecalciferol, Mass spectrometry, Neonates, Retinol, Umbilical cord blood diluted plasma",
author = "Albarhani, {Ali A.} and Fiona Collier and Greaves, {Ronda F.} and Ponsonby, {Anne Louise} and Allen, {Katrina J.} and Vuillermin, {Peter J.} and Peter Roche and Clarke, {Michael W.} and {BIS Steering Committee} and David Burgner and John Carlin and Richard Saffery",
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Albarhani, AA, Collier, F, Greaves, RF, Ponsonby, AL, Allen, KJ, Vuillermin, PJ, Roche, P, Clarke, MW & BIS Steering Committee 2015, 'Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma', Clinical Biochemistry, vol. 48, no. 16-17, pp. 1105-1112. https://doi.org/10.1016/j.clinbiochem.2015.04.014

Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma. / Albarhani, Ali A.; Collier, Fiona; Greaves, Ronda F.; Ponsonby, Anne Louise; Allen, Katrina J.; Vuillermin, Peter J.; Roche, Peter; Clarke, Michael W.; BIS Steering Committee.

In: Clinical Biochemistry, Vol. 48, No. 16-17, 16.01.2015, p. 1105-1112.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

AU - Albarhani, Ali A.

AU - Collier, Fiona

AU - Greaves, Ronda F.

AU - Ponsonby, Anne Louise

AU - Allen, Katrina J.

AU - Vuillermin, Peter J.

AU - Roche, Peter

AU - Clarke, Michael W.

AU - BIS Steering Committee

AU - Burgner, David

AU - Carlin, John

AU - Saffery, Richard

PY - 2015/1/16

Y1 - 2015/1/16

N2 - Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14. nmol/L [95% confidence interval (95% CI), - 6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2. nmol/L [95% CI, - 9.5 to 13.9] and 4.1. nmol/L [95% CI, - 14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07. μmol/L [95% CI, - 0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.

AB - Objectives: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. Design & methods: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. Results: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14. nmol/L [95% confidence interval (95% CI), - 6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2. nmol/L [95% CI, - 9.5 to 13.9] and 4.1. nmol/L [95% CI, - 14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07. μmol/L [95% CI, - 0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. Conclusions: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.

KW - Cholecalciferol

KW - Mass spectrometry

KW - Neonates

KW - Retinol

KW - Umbilical cord blood diluted plasma

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U2 - 10.1016/j.clinbiochem.2015.04.014

DO - 10.1016/j.clinbiochem.2015.04.014

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EP - 1112

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JF - Clinical Biochemistry

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Albarhani AA, Collier F, Greaves RF, Ponsonby AL, Allen KJ, Vuillermin PJ et al. Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma. Clinical Biochemistry. 2015 Jan 16;48(16-17):1105-1112. https://doi.org/10.1016/j.clinbiochem.2015.04.014