Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions

Kornél Nagy, Beatriz Lobo, Marie Claude Courtet-Compondu, Sophie Braga-Lagache, Laura Ramos, Valentí Puig-Divi, Fernando Azpiroz, Juan Ramón Malagelada, Maurice Beaumont, Julie Moulin, Simone Acquistapacea, Laurent Sagalowicz, Martin Kussmann, Javier Santos, Birgit Holst, Gary Williamson

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-(2H 6)]- a-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-2H3]-α-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography-mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached C max (0.6-4.6 μM depending on subject) in the 7-9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30-50% of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38-45%). No significant discrimination was found between the absorption of free or acetylated forms of deuterated a-tocopherol confirming that application of acetylated a-tocopherol provides the same bioavailability as free a-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-underthe- curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbatebased micelles cannot be generated.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalChimia
Volume68
Issue number3
DOIs
Publication statusPublished - 1 Mar 2014
Externally publishedYes

Keywords

  • Bioavailability
  • Chronic pancreas insufficiency
  • Self-assembly systems
  • Vitamin E

Cite this

Nagy, K., Lobo, B., Courtet-Compondu, M. C., Braga-Lagache, S., Ramos, L., Puig-Divi, V., ... Williamson, G. (2014). Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions. Chimia, 68(3), 129-134. https://doi.org/10.2533/chimia.2014.129
Nagy, Kornél ; Lobo, Beatriz ; Courtet-Compondu, Marie Claude ; Braga-Lagache, Sophie ; Ramos, Laura ; Puig-Divi, Valentí ; Azpiroz, Fernando ; Malagelada, Juan Ramón ; Beaumont, Maurice ; Moulin, Julie ; Acquistapacea, Simone ; Sagalowicz, Laurent ; Kussmann, Martin ; Santos, Javier ; Holst, Birgit ; Williamson, Gary. / Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions. In: Chimia. 2014 ; Vol. 68, No. 3. pp. 129-134.
@article{fc08e6548891417185039c555df95fba,
title = "Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions",
abstract = "We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-(2H 6)]- a-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-2H3]-α-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography-mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached C max (0.6-4.6 μM depending on subject) in the 7-9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30-50{\%} of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38-45{\%}). No significant discrimination was found between the absorption of free or acetylated forms of deuterated a-tocopherol confirming that application of acetylated a-tocopherol provides the same bioavailability as free a-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-underthe- curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbatebased micelles cannot be generated.",
keywords = "Bioavailability, Chronic pancreas insufficiency, Self-assembly systems, Vitamin E",
author = "Korn{\'e}l Nagy and Beatriz Lobo and Courtet-Compondu, {Marie Claude} and Sophie Braga-Lagache and Laura Ramos and Valent{\'i} Puig-Divi and Fernando Azpiroz and Malagelada, {Juan Ram{\'o}n} and Maurice Beaumont and Julie Moulin and Simone Acquistapacea and Laurent Sagalowicz and Martin Kussmann and Javier Santos and Birgit Holst and Gary Williamson",
year = "2014",
month = "3",
day = "1",
doi = "10.2533/chimia.2014.129",
language = "English",
volume = "68",
pages = "129--134",
journal = "Chimia",
issn = "0009-4293",
publisher = "Schweizerische Chemische Gesellschaft",
number = "3",

}

Nagy, K, Lobo, B, Courtet-Compondu, MC, Braga-Lagache, S, Ramos, L, Puig-Divi, V, Azpiroz, F, Malagelada, JR, Beaumont, M, Moulin, J, Acquistapacea, S, Sagalowicz, L, Kussmann, M, Santos, J, Holst, B & Williamson, G 2014, 'Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions', Chimia, vol. 68, no. 3, pp. 129-134. https://doi.org/10.2533/chimia.2014.129

Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions. / Nagy, Kornél; Lobo, Beatriz; Courtet-Compondu, Marie Claude; Braga-Lagache, Sophie; Ramos, Laura; Puig-Divi, Valentí; Azpiroz, Fernando; Malagelada, Juan Ramón; Beaumont, Maurice; Moulin, Julie; Acquistapacea, Simone; Sagalowicz, Laurent; Kussmann, Martin; Santos, Javier; Holst, Birgit; Williamson, Gary.

In: Chimia, Vol. 68, No. 3, 01.03.2014, p. 129-134.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions

AU - Nagy, Kornél

AU - Lobo, Beatriz

AU - Courtet-Compondu, Marie Claude

AU - Braga-Lagache, Sophie

AU - Ramos, Laura

AU - Puig-Divi, Valentí

AU - Azpiroz, Fernando

AU - Malagelada, Juan Ramón

AU - Beaumont, Maurice

AU - Moulin, Julie

AU - Acquistapacea, Simone

AU - Sagalowicz, Laurent

AU - Kussmann, Martin

AU - Santos, Javier

AU - Holst, Birgit

AU - Williamson, Gary

PY - 2014/3/1

Y1 - 2014/3/1

N2 - We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-(2H 6)]- a-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-2H3]-α-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography-mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached C max (0.6-4.6 μM depending on subject) in the 7-9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30-50% of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38-45%). No significant discrimination was found between the absorption of free or acetylated forms of deuterated a-tocopherol confirming that application of acetylated a-tocopherol provides the same bioavailability as free a-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-underthe- curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbatebased micelles cannot be generated.

AB - We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-(2H 6)]- a-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-2H3]-α-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography-mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached C max (0.6-4.6 μM depending on subject) in the 7-9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30-50% of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38-45%). No significant discrimination was found between the absorption of free or acetylated forms of deuterated a-tocopherol confirming that application of acetylated a-tocopherol provides the same bioavailability as free a-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-underthe- curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbatebased micelles cannot be generated.

KW - Bioavailability

KW - Chronic pancreas insufficiency

KW - Self-assembly systems

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=84898717555&partnerID=8YFLogxK

U2 - 10.2533/chimia.2014.129

DO - 10.2533/chimia.2014.129

M3 - Article

VL - 68

SP - 129

EP - 134

JO - Chimia

JF - Chimia

SN - 0009-4293

IS - 3

ER -

Nagy K, Lobo B, Courtet-Compondu MC, Braga-Lagache S, Ramos L, Puig-Divi V et al. Vitamine and vitamine acetate absorption from self-assembly systems under pancreas insufficiency conditions. Chimia. 2014 Mar 1;68(3):129-134. https://doi.org/10.2533/chimia.2014.129