Vital organ blood flow during hyperdynamic sepsis

David Di Giantomasso, Clive N. May, Rinaldo Bellomo

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137 Citations (Scopus)

Abstract

Objectives: To develop a nonlethal model of hyperdynamic sepsis, and to measure vital organ blood flows in this setting. Design: Randomized crossover animal study. Setting: Animal laboratory of university-affiliated physiology institute. Subjects: Seven Merino cross sheep. Interventions: Surgical implantation of transit-time flow probes around sagittal sinus and circumflex coronary, superior mesenteric, and left renal arteries, and of an electromagnetic flow probe around the ascending aorta. After recovery, randomization to either 6 h of observation under normal conditions (control) or 6 h of observation after the induction of hyperdynamic nonlethal sepsis (sepsis), with each animal crossing over to the other treatment after a 2-week interval. Measurements and main results: Injection of Escherichia coli induced nonlethal hyperdynamic sepsis within 5 to 6 h with hypotension (mean arterial pressure [± SD], 85 ± 7 mm Hg vs 69 ± 8 mm Hg), increased cardiac output (4.0 ± 0.9 L/min vs 7.2 ± 1.2 L/min), tachycardia (60 ± 10 beats/min vs 160 ± 15 beats/min), fever, oliguria, and tachypnea. Compared to control animals, hyperdynamic sepsis increased renal (330 ± 101 mL/min vs 214 ± 75 mL/min), mesenteric (773 ± 370 mL/min vs 516 ± 221 mL/min), and coronary (54 ± 24 mL/min vs 23 ± 10 mL/min) blood flow (p < 0.05). There was no significant change in sagittal sinus flow. Despite increased coronary, flow, myocardial contractility decreased (800 ± 150 L/min/s vs 990 ± 150 L/min/s). Despite increased mesenteric and renal blood flow, there was hyperlactatemia (0.5 ± 0.1 mmol/L vs 1.9 ± 0.3 mmol/L); despite increased renal blood flow, all experimental animals acquired oliguria (160 ± 75.3 mL/2 h vs 50.2 ± 13.1 mL/2 h) and increased serum creatinine levels (0.07 ± 0.02 mmol/L vs 0.11 ± 0.02 mmol/L). Conclusions: Injection of E coli induced hyperdynamic nonlethal sepsis. During such hyperdynamic sepsis, blood flow to heart, gut, and kidney was markedly increased; however, organ dysfunction developed. We speculate that global ischemia may not be the principal mechanism of vital organ dysfunction in hyperdynamic sepsis.

Original languageEnglish
Pages (from-to)1053-1059
Number of pages7
JournalChest
Volume124
Issue number3
DOIs
Publication statusPublished - 1 Sep 2003
Externally publishedYes

Keywords

  • Blood flow
  • Brain
  • Cerebral circulation
  • Coronary circulation
  • Gut
  • Heart
  • Kidney
  • Sepsis

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