Visualizing cancer cell chemotaxis and invasion in 2D and 3D

Olivia Susanto, Andrew J. Muinonen-Martin, Max Nobis, Robert H. Insall

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

4 Citations (Scopus)

Abstract

We describe three chemotaxis assays—Insall chambers, circular invasion assays, and 3D organotypic assays— that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsTian Jin, Dale Hereld
Place of PublicationNew York, NY
PublisherHumana Press
Chapter17
Pages217-228
Number of pages12
ISBN (Electronic)9781493934805
ISBN (Print)9781493934782
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1407
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell migration
  • Chemotaxis
  • Invasion
  • Melanoma
  • Metastasis
  • Organotypic

Cite this

Susanto, O., Muinonen-Martin, A. J., Nobis, M., & Insall, R. H. (2016). Visualizing cancer cell chemotaxis and invasion in 2D and 3D. In T. Jin, & D. Hereld (Eds.), Methods in Molecular Biology (pp. 217-228). (Methods in Molecular Biology; Vol. 1407). Humana Press. https://doi.org/10.1007/978-1-4939-3480-5_17