Visualization of fluorescent protein expression in whole-mount postimplantation-stage mouse embryos

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

Abstract

Fluorescent protein (FP)-expressing transgenic mice are powerful genetic resources for marking both live and fixed cells and tissues. Expression in live embryos requires only dissection and visualization using an appropriate microscope. Fixation can compromise FP activity. A simple tissue-clearing agent called Scale preserves FP activity while rendering the embryo or organ optically transparent.

Original languageEnglish
Pages (from-to)171-172
Number of pages2
JournalCold Spring Harbor Protocols
Volume2018
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Cite this

Behringer, Richard ; Gertsenstein, Marina ; Nagy, Kristina Vintersten ; Nagy, Andras. / Visualization of fluorescent protein expression in whole-mount postimplantation-stage mouse embryos. In: Cold Spring Harbor Protocols. 2018 ; Vol. 2018, No. 3. pp. 171-172.
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Visualization of fluorescent protein expression in whole-mount postimplantation-stage mouse embryos. / Behringer, Richard; Gertsenstein, Marina; Nagy, Kristina Vintersten; Nagy, Andras.

In: Cold Spring Harbor Protocols, Vol. 2018, No. 3, 01.03.2018, p. 171-172.

Research output: Contribution to journalArticleOtherpeer-review

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