Visualization of CombiHIVvac vaccine particles using electron microscopy

Larisa I. Karpenko, Leonid R. Lebedev, Sergei I. Bazhan, Denis V. Korneev, Boris B. Zaitsev, Alexander A. Ilyichev

Research output: Contribution to journalShort SurveyOtherpeer-review

Abstract

A candidate vaccine CombiHIVvac is developed; presently the clinical phase I trial has been completed successfully. CombiHIVvac combines the conserved polyepitope immunogens approaches in a novel self-adjuvanted microparticle concept. The artificial TBI (T cell and B cell immunogen) polypeptide used in the vaccine comprises epitopes from Env and Gag. The polypeptide is conjugated to dextran and mixed with DNA, which leads to formation of microparticles presenting TBI on the surface and containing the DNA inside. The DNA (pcDNA-TCI) enclosed in the microparticles codes for the TCI (T cell immunogen) polypeptide, which contains CD8+ and CD4+ epitopes from Env, Gag, Pol, and Nef conserved among HIV subtypes A, B, and C.1,2 The proposed technique enables the vaccine components to combine into particles on the principle of self-assembly (Fig. 1A).
Original languageEnglish
Pages (from-to)323-324
Number of pages2
JournalAIDS Research and Human Retroviruses
Volume33
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

Keywords

  • Artificial proteins
  • DNA vaccine
  • HIV vaccine
  • Phase I clinical trials
  • Polyepitope immunogens
  • Self-assemble microparticles

Cite this

Karpenko, L. I., Lebedev, L. R., Bazhan, S. I., Korneev, D. V., Zaitsev, B. B., & Ilyichev, A. A. (2017). Visualization of CombiHIVvac vaccine particles using electron microscopy. AIDS Research and Human Retroviruses, 33(4), 323-324. https://doi.org/10.1089/aid.2016.0140
Karpenko, Larisa I. ; Lebedev, Leonid R. ; Bazhan, Sergei I. ; Korneev, Denis V. ; Zaitsev, Boris B. ; Ilyichev, Alexander A. / Visualization of CombiHIVvac vaccine particles using electron microscopy. In: AIDS Research and Human Retroviruses. 2017 ; Vol. 33, No. 4. pp. 323-324.
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Karpenko, LI, Lebedev, LR, Bazhan, SI, Korneev, DV, Zaitsev, BB & Ilyichev, AA 2017, 'Visualization of CombiHIVvac vaccine particles using electron microscopy', AIDS Research and Human Retroviruses, vol. 33, no. 4, pp. 323-324. https://doi.org/10.1089/aid.2016.0140

Visualization of CombiHIVvac vaccine particles using electron microscopy. / Karpenko, Larisa I.; Lebedev, Leonid R.; Bazhan, Sergei I.; Korneev, Denis V.; Zaitsev, Boris B.; Ilyichev, Alexander A.

In: AIDS Research and Human Retroviruses, Vol. 33, No. 4, 01.04.2017, p. 323-324.

Research output: Contribution to journalShort SurveyOtherpeer-review

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T1 - Visualization of CombiHIVvac vaccine particles using electron microscopy

AU - Karpenko, Larisa I.

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AU - Zaitsev, Boris B.

AU - Ilyichev, Alexander A.

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AB - A candidate vaccine CombiHIVvac is developed; presently the clinical phase I trial has been completed successfully. CombiHIVvac combines the conserved polyepitope immunogens approaches in a novel self-adjuvanted microparticle concept. The artificial TBI (T cell and B cell immunogen) polypeptide used in the vaccine comprises epitopes from Env and Gag. The polypeptide is conjugated to dextran and mixed with DNA, which leads to formation of microparticles presenting TBI on the surface and containing the DNA inside. The DNA (pcDNA-TCI) enclosed in the microparticles codes for the TCI (T cell immunogen) polypeptide, which contains CD8+ and CD4+ epitopes from Env, Gag, Pol, and Nef conserved among HIV subtypes A, B, and C.1,2 The proposed technique enables the vaccine components to combine into particles on the principle of self-assembly (Fig. 1A).

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