Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Chai Hoon Khoo, Yoke Kqueen Cheah, Learn Han Lee, Jiun Horng Sim, Noorzaleha Awang Salleh, Shiran Mohd Sidik, Son Radu, Sabrina Sukardi

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)

Abstract

The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg μl-1. This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies.

Original languageEnglish
Pages (from-to)441-457
Number of pages17
JournalAntonie Van Leeuwenhoek
Volume96
Issue number4
DOIs
Publication statusPublished - Nov 2009
Externally publishedYes

Keywords

  • Multiplex PCR
  • Salmonella enterica
  • Salmonella pathogenicity islands (SPIs)
  • Virulotyping

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