TY - JOUR
T1 - Virological and immunological outcomes in rhesus monkeys after exposure to dengue virus-infected Aedes aegypti mosquitoes
AU - Carrington, Lauren B.
AU - Ponlawat, Alongkot
AU - Nitatsukprasert, Chanyapat
AU - Khongtak, Patcharee
AU - Sunyakumthorn, Piyanate
AU - Ege, Christine A.
AU - Im-Erbsin, Rawiwan
AU - Chumpolkulwong, Kesara
AU - Thaisomboonsuk, Butsaya
AU - Klungthong, Chonticha
AU - Yoon, In-Kyu
AU - Ellison, Damon
AU - Macareo, Louis
AU - Simmons, Cameron P.
N1 - Funding Information:
Financial support: This work was funded by a Wellcome Trust grant to CPS.
Publisher Copyright:
© 2020 American Society of Tropical Medicine and Hygiene. All rights reserved.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - This study describes the natural history of dengue virus (DENV) infection in rhesus monkeys exposed to the bites of DENV-infected Aedes aegypti mosquitoes. Dengue virus-infected mosquitoes were generated by either intrathoracic inoculation or by oral feeding on viremic blood meals. Each of the six rhesus monkeys that were fed upon by intrathoracically infected mosquitoes developed non-structural protein 1 (NS1) antigenemia and an IgM response; viremia was detected in 4/6 individuals. No virological or immunological evidence of DENV infection was detected in the three monkeys exposed to mosquitoes that had been orally infected with DENV. These results demonstrate the utility of mosquito-borne challenge of rhesus monkeys with DENV.
AB - This study describes the natural history of dengue virus (DENV) infection in rhesus monkeys exposed to the bites of DENV-infected Aedes aegypti mosquitoes. Dengue virus-infected mosquitoes were generated by either intrathoracic inoculation or by oral feeding on viremic blood meals. Each of the six rhesus monkeys that were fed upon by intrathoracically infected mosquitoes developed non-structural protein 1 (NS1) antigenemia and an IgM response; viremia was detected in 4/6 individuals. No virological or immunological evidence of DENV infection was detected in the three monkeys exposed to mosquitoes that had been orally infected with DENV. These results demonstrate the utility of mosquito-borne challenge of rhesus monkeys with DENV.
UR - http://www.scopus.com/inward/record.url?scp=85088206104&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.19-0633
DO - 10.4269/ajtmh.19-0633
M3 - Article
C2 - 32431270
AN - SCOPUS:85088206104
SN - 0002-9637
VL - 103
SP - 112
EP - 119
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 1
ER -
