Abstract
Recent developments in photochemistry have introduced new methods to prepare hydrogels initiated by nonharmful light which is essential for encapsulation of cells and bioactive components. However, bioorthogonal photoclick reactions generally requires two components for cross-linking and, in many cases, the formation of a reactive intermediate that may cross-react with nucleophiles in biological media. Here we report the utilization of a visible light triggered dimerization of electron-rich anthracene for polymer cross-linking to form bulk hydrogels and microgels. Incorporation of gelatin within the hydrogel enhanced cell attachment and viability after 7 days of culture and spatiotemporal conjugation of a bioactive component using photochemical dimerization of anthracene was demonstrated. This work therefore introduces a simple yet powerful tool for light modulated bioorthogonal polymer cross-linking, which can be utilized in various bioengineering applications.
Original language | English |
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Pages (from-to) | 657-662 |
Number of pages | 6 |
Journal | ACS Macro Letters |
Volume | 6 |
Issue number | 7 |
DOIs | |
Publication status | Published - 18 Jul 2017 |
Equipment
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Monash Micro Imaging
Stephen Firth (Manager), Alex Fulcher (Operator), Oleks Chernyavskiy (Operator), Margaret Rzeszutek (Other), David Potter (Manager), Volker Hilsenstein (Operator), Juan Nunez-Iglesias (Other), Stephen Cody (Manager), Irena Carmichael (Operator), Betty Kouskousis (Other), Sarah Creed (Manager) & Giulia Ballerin (Operator)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility