Abstract
| Original language | English |
|---|---|
| Pages (from-to) | L213 - L223 |
| Number of pages | 11 |
| Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
| Volume | 310 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2016 |
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Ventilation-induced lung injury is not exacerbated by growth restriction in preterm lambs. / Allison, Beth J.; Hooper, Stuart B.; Coia, Elise; Zahra, Valerie A.; Jenkin, Graham; Malhotra, Atul; Sehgal, Arvind; Kluckow, Martin; Gill, Andrew W.; Sozo, Foula; Miller, Suzanne L.; Polglase, Graeme R.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 310, No. 3, 2016, p. L213 - L223.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Ventilation-induced lung injury is not exacerbated by growth restriction in preterm lambs
AU - Allison, Beth J.
AU - Hooper, Stuart B.
AU - Coia, Elise
AU - Zahra, Valerie A.
AU - Jenkin, Graham
AU - Malhotra, Atul
AU - Sehgal, Arvind
AU - Kluckow, Martin
AU - Gill, Andrew W.
AU - Sozo, Foula
AU - Miller, Suzanne L.
AU - Polglase, Graeme R.
PY - 2016
Y1 - 2016
N2 - Intrauterine growth restriction (IUGR) and preterm birth are frequent co-morbidities and, combined, increase the risk of adverse respiratory outcomes compared to that in appropriately grown infants. Potential underlying reasons for this increased respiratory morbidity in IUGR infants compared to appropriately grown infants include altered fetal lung development, fetal lung inflammation, increased respiratory requirements and/or increased ventilation induced lung injury Methods: IUGR was surgically induced in preterm fetal sheep (0.7 gestation) by ligation of a single umbilical artery. Four weeks later, preterm lambs were euthanized at delivery or delivered and ventilated for 2 hours before euthanasia. Ventilator requirements, lung inflammation, early markers of lung injury and morphological changes in lung parenchymal and vascular structure and surfactant composition were analyzed. RESULTS: IUGR preterm lambs weighed 30 less than appropriately grown (AG) preterm lambs, with increased brain:body weight ratio, indicating brain sparing. IUGR did not induce lung inflammation or injury or alter lung parenchymal and vascular structure compared to AG fetuses. IUGR and AG lambs had similar oxygenation and respiratory requirements after birth, and had significant, but similar, increases in pro-inflammatory cytokine expression, lung injury markers gene expression and surfactant phosphatidylcholine species compared to unventilated controls. CONCLUSION: IUGR does not induce pulmonary structural changes in our model. Furthermore, IUGR and AG preterm lambs have similar ventilator requirements in the immediate postnatal period. This study suggests that increased morbidity and mortality in IUGR infants is not due to altered lung tissue or vascular structure, or to an altered response to early ventilation.
AB - Intrauterine growth restriction (IUGR) and preterm birth are frequent co-morbidities and, combined, increase the risk of adverse respiratory outcomes compared to that in appropriately grown infants. Potential underlying reasons for this increased respiratory morbidity in IUGR infants compared to appropriately grown infants include altered fetal lung development, fetal lung inflammation, increased respiratory requirements and/or increased ventilation induced lung injury Methods: IUGR was surgically induced in preterm fetal sheep (0.7 gestation) by ligation of a single umbilical artery. Four weeks later, preterm lambs were euthanized at delivery or delivered and ventilated for 2 hours before euthanasia. Ventilator requirements, lung inflammation, early markers of lung injury and morphological changes in lung parenchymal and vascular structure and surfactant composition were analyzed. RESULTS: IUGR preterm lambs weighed 30 less than appropriately grown (AG) preterm lambs, with increased brain:body weight ratio, indicating brain sparing. IUGR did not induce lung inflammation or injury or alter lung parenchymal and vascular structure compared to AG fetuses. IUGR and AG lambs had similar oxygenation and respiratory requirements after birth, and had significant, but similar, increases in pro-inflammatory cytokine expression, lung injury markers gene expression and surfactant phosphatidylcholine species compared to unventilated controls. CONCLUSION: IUGR does not induce pulmonary structural changes in our model. Furthermore, IUGR and AG preterm lambs have similar ventilator requirements in the immediate postnatal period. This study suggests that increased morbidity and mortality in IUGR infants is not due to altered lung tissue or vascular structure, or to an altered response to early ventilation.
UR - http://ajplung.physiology.org/content/ajplung/310/3/L213.full.pdf
U2 - 10.1152/ajplung.00328.2015
DO - 10.1152/ajplung.00328.2015
M3 - Article
VL - 310
SP - L213 - L223
JO - American Journal of Physiology - Lung Cellular and Molecular Physiology
JF - American Journal of Physiology - Lung Cellular and Molecular Physiology
SN - 1040-0605
IS - 3
ER -