Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 310 - 316 |
| Number of pages | 7 |
| Journal | Pediatric Research |
| Volume | 73 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2013 |
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VEGF expression and microvascular responses to severe transient hypoxia in the fetal sheep brain. / Baburamani, Ana Aradhna; Zakhem, Margie Esmeralda; Walker, David William.
In: Pediatric Research, Vol. 73, No. 3, 2013, p. 310 - 316.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - VEGF expression and microvascular responses to severe transient hypoxia in the fetal sheep brain
AU - Baburamani, Ana Aradhna
AU - Zakhem, Margie Esmeralda
AU - Walker, David William
PY - 2013
Y1 - 2013
N2 - BACKGROUND: Fetal hypoxia contributes significantly to the pathogenesis of permanent perinatal brain injury. We hypothesized that hypoxia-induced cerebral angiogenesis and microvascular changes would occur in fetal sheep subjected to a severe hypoxic insult produced by umbilical cord occlusion (UCO) for 10 min. METHODS: At 124-126 d of gestation, singleton fetal sheep underwent surgery for implantation of catheters and placement of an inflatable cuff around the umbilical cord. A 10-min UCO or sham UCO (n = 5) was induced at 130 d gestation. The fetal brain was collected at 24 h (n = 5) or 48 h (n = 4) after UCO for immunohistochemical analysis of vascular endothelial growth factor (VEGF), Ki67, and serum albumin. RESULTS: By 48 h after UCO, the percentage of blood vessels expressing VEGF had increased in the subventricular zone, periventricular and subcortical white matter, corpus callosum, and cortex. Alterations in vascular permeability (albumin extravasation) were observed only in the periventricular and subcortical white matter and the subventricular zone following UCO. CONCLUSION: The upregulation of VEGF expression and increased leakage of plasma protein in the fetal sheep brain show that the microvasculature in white matter is sensitive to hypoxia in the near-term brain.
AB - BACKGROUND: Fetal hypoxia contributes significantly to the pathogenesis of permanent perinatal brain injury. We hypothesized that hypoxia-induced cerebral angiogenesis and microvascular changes would occur in fetal sheep subjected to a severe hypoxic insult produced by umbilical cord occlusion (UCO) for 10 min. METHODS: At 124-126 d of gestation, singleton fetal sheep underwent surgery for implantation of catheters and placement of an inflatable cuff around the umbilical cord. A 10-min UCO or sham UCO (n = 5) was induced at 130 d gestation. The fetal brain was collected at 24 h (n = 5) or 48 h (n = 4) after UCO for immunohistochemical analysis of vascular endothelial growth factor (VEGF), Ki67, and serum albumin. RESULTS: By 48 h after UCO, the percentage of blood vessels expressing VEGF had increased in the subventricular zone, periventricular and subcortical white matter, corpus callosum, and cortex. Alterations in vascular permeability (albumin extravasation) were observed only in the periventricular and subcortical white matter and the subventricular zone following UCO. CONCLUSION: The upregulation of VEGF expression and increased leakage of plasma protein in the fetal sheep brain show that the microvasculature in white matter is sensitive to hypoxia in the near-term brain.
UR - http://www.nature.com/pr/journal/v73/n3/pdf/pr2012191a.pdf
U2 - 10.1038/pr.2012.191
DO - 10.1038/pr.2012.191
M3 - Article
VL - 73
SP - 310
EP - 316
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 3
ER -