Vampire venom

Vasodilatory mechanisms of vampire bat (desmodus rotundus) blood feeding

Rahini Kakumanu, Wayne C. Hodgson, Ravina Ravi, Alejandro Alagon, Richard J. Harris, Andreas Brust, Paul F. Alewood, Barbara K. Kemp-Harper, Bryan G. Fry

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Animals that specialise in blood feeding have particular challenges in obtaining their meal, whereby they impair blood hemostasis by promoting anticoagulation and vasodilation in order to facilitate feeding. These convergent selection pressures have been studied in a number of lineages, ranging from fleas to leeches. However, the vampire bat (Desmondus rotundus) is unstudied in regards to potential vasodilatory mechanisms of their feeding secretions (which are a type of venom). This is despite the intense investigations of their anticoagulant properties which have demonstrated that D. rotundus venom contains strong anticoagulant and proteolytic activities which delay the formation of blood clots and interfere with the blood coagulation cascade. In this study, we identified and tested a compound from D. rotundus venom that is similar in size and amino acid sequence to human calcitonin gene-related peptide (CGRP) which has potent vasodilatory properties. We found that the vampire bat-derived form of CGRP (i.e., vCGRP) selectively caused endothelium-independent relaxation of pre-contracted rat small mesenteric arteries. The vasorelaxant efficacy and potency of vCGRP were similar to that of CGRP, in activating CGRP receptors and Kv channels to relax arteriole smooth muscle, which would facilitate blood meal feeding by promoting continual blood flow. Our results provide, for the first time, a detailed investigation into the identification and function of a vasodilatory peptide found in D. rotundus venom, which provides a basis in understanding the convergent pathways and selectivity of hematophagous venoms. These unique peptides also show excellent drug design and development potential, thus highlighting the social and economic value of venomous animals.

Original languageEnglish
Article number26
Number of pages10
JournalToxins
Volume11
Issue number1
DOIs
Publication statusPublished - 8 Jan 2019

Keywords

  • Calcitonin gene-related peptide
  • Desmodus rotundus
  • Potassium channels
  • Vampire bat
  • Vasodilatation
  • Venom

Cite this

Kakumanu, R., Hodgson, W. C., Ravi, R., Alagon, A., Harris, R. J., Brust, A., ... Fry, B. G. (2019). Vampire venom: Vasodilatory mechanisms of vampire bat (desmodus rotundus) blood feeding. Toxins, 11(1), [26]. https://doi.org/10.3390/toxins11010026
Kakumanu, Rahini ; Hodgson, Wayne C. ; Ravi, Ravina ; Alagon, Alejandro ; Harris, Richard J. ; Brust, Andreas ; Alewood, Paul F. ; Kemp-Harper, Barbara K. ; Fry, Bryan G. / Vampire venom : Vasodilatory mechanisms of vampire bat (desmodus rotundus) blood feeding. In: Toxins. 2019 ; Vol. 11, No. 1.
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abstract = "Animals that specialise in blood feeding have particular challenges in obtaining their meal, whereby they impair blood hemostasis by promoting anticoagulation and vasodilation in order to facilitate feeding. These convergent selection pressures have been studied in a number of lineages, ranging from fleas to leeches. However, the vampire bat (Desmondus rotundus) is unstudied in regards to potential vasodilatory mechanisms of their feeding secretions (which are a type of venom). This is despite the intense investigations of their anticoagulant properties which have demonstrated that D. rotundus venom contains strong anticoagulant and proteolytic activities which delay the formation of blood clots and interfere with the blood coagulation cascade. In this study, we identified and tested a compound from D. rotundus venom that is similar in size and amino acid sequence to human calcitonin gene-related peptide (CGRP) which has potent vasodilatory properties. We found that the vampire bat-derived form of CGRP (i.e., vCGRP) selectively caused endothelium-independent relaxation of pre-contracted rat small mesenteric arteries. The vasorelaxant efficacy and potency of vCGRP were similar to that of CGRP, in activating CGRP receptors and Kv channels to relax arteriole smooth muscle, which would facilitate blood meal feeding by promoting continual blood flow. Our results provide, for the first time, a detailed investigation into the identification and function of a vasodilatory peptide found in D. rotundus venom, which provides a basis in understanding the convergent pathways and selectivity of hematophagous venoms. These unique peptides also show excellent drug design and development potential, thus highlighting the social and economic value of venomous animals.",
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Kakumanu, R, Hodgson, WC, Ravi, R, Alagon, A, Harris, RJ, Brust, A, Alewood, PF, Kemp-Harper, BK & Fry, BG 2019, 'Vampire venom: Vasodilatory mechanisms of vampire bat (desmodus rotundus) blood feeding', Toxins, vol. 11, no. 1, 26. https://doi.org/10.3390/toxins11010026

Vampire venom : Vasodilatory mechanisms of vampire bat (desmodus rotundus) blood feeding. / Kakumanu, Rahini; Hodgson, Wayne C.; Ravi, Ravina; Alagon, Alejandro; Harris, Richard J.; Brust, Andreas; Alewood, Paul F.; Kemp-Harper, Barbara K.; Fry, Bryan G.

In: Toxins, Vol. 11, No. 1, 26, 08.01.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Vampire venom

T2 - Vasodilatory mechanisms of vampire bat (desmodus rotundus) blood feeding

AU - Kakumanu, Rahini

AU - Hodgson, Wayne C.

AU - Ravi, Ravina

AU - Alagon, Alejandro

AU - Harris, Richard J.

AU - Brust, Andreas

AU - Alewood, Paul F.

AU - Kemp-Harper, Barbara K.

AU - Fry, Bryan G.

PY - 2019/1/8

Y1 - 2019/1/8

N2 - Animals that specialise in blood feeding have particular challenges in obtaining their meal, whereby they impair blood hemostasis by promoting anticoagulation and vasodilation in order to facilitate feeding. These convergent selection pressures have been studied in a number of lineages, ranging from fleas to leeches. However, the vampire bat (Desmondus rotundus) is unstudied in regards to potential vasodilatory mechanisms of their feeding secretions (which are a type of venom). This is despite the intense investigations of their anticoagulant properties which have demonstrated that D. rotundus venom contains strong anticoagulant and proteolytic activities which delay the formation of blood clots and interfere with the blood coagulation cascade. In this study, we identified and tested a compound from D. rotundus venom that is similar in size and amino acid sequence to human calcitonin gene-related peptide (CGRP) which has potent vasodilatory properties. We found that the vampire bat-derived form of CGRP (i.e., vCGRP) selectively caused endothelium-independent relaxation of pre-contracted rat small mesenteric arteries. The vasorelaxant efficacy and potency of vCGRP were similar to that of CGRP, in activating CGRP receptors and Kv channels to relax arteriole smooth muscle, which would facilitate blood meal feeding by promoting continual blood flow. Our results provide, for the first time, a detailed investigation into the identification and function of a vasodilatory peptide found in D. rotundus venom, which provides a basis in understanding the convergent pathways and selectivity of hematophagous venoms. These unique peptides also show excellent drug design and development potential, thus highlighting the social and economic value of venomous animals.

AB - Animals that specialise in blood feeding have particular challenges in obtaining their meal, whereby they impair blood hemostasis by promoting anticoagulation and vasodilation in order to facilitate feeding. These convergent selection pressures have been studied in a number of lineages, ranging from fleas to leeches. However, the vampire bat (Desmondus rotundus) is unstudied in regards to potential vasodilatory mechanisms of their feeding secretions (which are a type of venom). This is despite the intense investigations of their anticoagulant properties which have demonstrated that D. rotundus venom contains strong anticoagulant and proteolytic activities which delay the formation of blood clots and interfere with the blood coagulation cascade. In this study, we identified and tested a compound from D. rotundus venom that is similar in size and amino acid sequence to human calcitonin gene-related peptide (CGRP) which has potent vasodilatory properties. We found that the vampire bat-derived form of CGRP (i.e., vCGRP) selectively caused endothelium-independent relaxation of pre-contracted rat small mesenteric arteries. The vasorelaxant efficacy and potency of vCGRP were similar to that of CGRP, in activating CGRP receptors and Kv channels to relax arteriole smooth muscle, which would facilitate blood meal feeding by promoting continual blood flow. Our results provide, for the first time, a detailed investigation into the identification and function of a vasodilatory peptide found in D. rotundus venom, which provides a basis in understanding the convergent pathways and selectivity of hematophagous venoms. These unique peptides also show excellent drug design and development potential, thus highlighting the social and economic value of venomous animals.

KW - Calcitonin gene-related peptide

KW - Desmodus rotundus

KW - Potassium channels

KW - Vampire bat

KW - Vasodilatation

KW - Venom

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