Validation and optimization of a simple RP-HPLC method for determination of trimetazidine in human serum and its application in a pharmacokinetic study with healthy Bangladeshi male volunteers

Md Mazharul Islam Chowdhury, Md Ashik Ullah, Abdullah Al Maruf, Mohammad Safiqul Islam, Maizbha Uddin Ahmed, Md Hasanuzzaman Shohag, M. A.K. Azad, Abul Hasnat

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6 Citations (Scopus)

Abstract

Trimetazidine is an effective and well-tolerated antianginal drug. In the present study, a simple, sensitive and specific liquid chromatography (HPLC) method with UV detection was developed and validated for the quantification of trimetazidine in human serum samples using caffeine as internal standard. Protein precipitation method with methanol was employed in the extraction of trimetazidine and caffeine from biological matrix. The chromatographic separation was accomplished on Xterra C 18 Column with a mobile phase consisting 0.01 M potassium dihydrogen phosphate buffer (pH 4.16 ± 0.01 adjusted with orthophosphoric acid, with a solvent system of triethanolamine and acetonitrile (90:10) at a flow rate of 1.0 ml/min. The chromatogram was monitored at a wavelength of 207 nm. The method was validated over a linear concentration range of 5-200 ng/ml and limit of quantification (LOQ) was 5.0 ng/ml with a coefficient of correlation (r 2) ≥ 0.996. The intra-day and inter-day precision expressed as relative standard deviation was 3.40%-11.63% and 1.30%-10.21%, respectively. The average recovery of trimetazidine from serum was 97.44%. The method was successfully applied to a pharmacokinetic study after oral administration of modified release trimetazidine hydrochloride tablet (35 mg) in healthy Bangladeshi volunteers.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalDhaka University Journal of Pharmaceutical Sciences
Volume10
Issue number2
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Keywords

  • Antianginal drug
  • Bangladeshi volunteer
  • Method development and validation
  • Pharmacokinetics
  • Trimetazidine

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