Vaginal concentrations of lactic acid potently inactivate HIV

Muriel Constanza Aldunate, David Tyssen, Adam Johnson, Tasnim Saifudin Zakir, Secondo Sonza, Thomas R Moench, Richard Cone, Gilda Tachedjian

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Abstract

When Lactobacillus spp. dominate the vaginal microbiota of women of reproductive age they acidify the vagina to pH /=0.4 (w/w) l-lactic acid. At a typical vaginal pH of 3.8, l-lactic acid at 1 (w/w) more potently and rapidly inactivated HIVBa-L and HIV-1 transmitter/founder strains compared with 1 (w/w) acetic acid and with acidity alone, all adjusted to pH 3.8. A final concentration of 1 (w/w) l-lactic acid maximally inactivated HIVBa-L in the presence of cervicovaginal secretions and seminal plasma. The anti-HIV activity of l-lactic acid was pH dependent, being abrogated at neutral pH, indicating that its virucidal activity is mediated by protonated lactic acid and not the lactate anion. CONCLUSIONS: l-lactic acid at physiological concentrations demonstrates potent HIV virucidal activity distinct from acidity alone and greater than acetic acid, suggesting a protective role in the sexual transmission of HIV.
Original languageEnglish
Pages (from-to)2015 - 2025
Number of pages11
JournalJournal of Antimicrobial Chemotherapy
Volume68
Issue number9
DOIs
Publication statusPublished - 2013

Cite this

Aldunate, Muriel Constanza ; Tyssen, David ; Johnson, Adam ; Zakir, Tasnim Saifudin ; Sonza, Secondo ; Moench, Thomas R ; Cone, Richard ; Tachedjian, Gilda. / Vaginal concentrations of lactic acid potently inactivate HIV. In: Journal of Antimicrobial Chemotherapy. 2013 ; Vol. 68, No. 9. pp. 2015 - 2025.
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title = "Vaginal concentrations of lactic acid potently inactivate HIV",
abstract = "When Lactobacillus spp. dominate the vaginal microbiota of women of reproductive age they acidify the vagina to pH /=0.4 (w/w) l-lactic acid. At a typical vaginal pH of 3.8, l-lactic acid at 1 (w/w) more potently and rapidly inactivated HIVBa-L and HIV-1 transmitter/founder strains compared with 1 (w/w) acetic acid and with acidity alone, all adjusted to pH 3.8. A final concentration of 1 (w/w) l-lactic acid maximally inactivated HIVBa-L in the presence of cervicovaginal secretions and seminal plasma. The anti-HIV activity of l-lactic acid was pH dependent, being abrogated at neutral pH, indicating that its virucidal activity is mediated by protonated lactic acid and not the lactate anion. CONCLUSIONS: l-lactic acid at physiological concentrations demonstrates potent HIV virucidal activity distinct from acidity alone and greater than acetic acid, suggesting a protective role in the sexual transmission of HIV.",
author = "Aldunate, {Muriel Constanza} and David Tyssen and Adam Johnson and Zakir, {Tasnim Saifudin} and Secondo Sonza and Moench, {Thomas R} and Richard Cone and Gilda Tachedjian",
year = "2013",
doi = "10.1093/jac/dkt156",
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Aldunate, MC, Tyssen, D, Johnson, A, Zakir, TS, Sonza, S, Moench, TR, Cone, R & Tachedjian, G 2013, 'Vaginal concentrations of lactic acid potently inactivate HIV', Journal of Antimicrobial Chemotherapy, vol. 68, no. 9, pp. 2015 - 2025. https://doi.org/10.1093/jac/dkt156

Vaginal concentrations of lactic acid potently inactivate HIV. / Aldunate, Muriel Constanza; Tyssen, David; Johnson, Adam; Zakir, Tasnim Saifudin; Sonza, Secondo; Moench, Thomas R; Cone, Richard; Tachedjian, Gilda.

In: Journal of Antimicrobial Chemotherapy, Vol. 68, No. 9, 2013, p. 2015 - 2025.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Vaginal concentrations of lactic acid potently inactivate HIV

AU - Aldunate, Muriel Constanza

AU - Tyssen, David

AU - Johnson, Adam

AU - Zakir, Tasnim Saifudin

AU - Sonza, Secondo

AU - Moench, Thomas R

AU - Cone, Richard

AU - Tachedjian, Gilda

PY - 2013

Y1 - 2013

N2 - When Lactobacillus spp. dominate the vaginal microbiota of women of reproductive age they acidify the vagina to pH /=0.4 (w/w) l-lactic acid. At a typical vaginal pH of 3.8, l-lactic acid at 1 (w/w) more potently and rapidly inactivated HIVBa-L and HIV-1 transmitter/founder strains compared with 1 (w/w) acetic acid and with acidity alone, all adjusted to pH 3.8. A final concentration of 1 (w/w) l-lactic acid maximally inactivated HIVBa-L in the presence of cervicovaginal secretions and seminal plasma. The anti-HIV activity of l-lactic acid was pH dependent, being abrogated at neutral pH, indicating that its virucidal activity is mediated by protonated lactic acid and not the lactate anion. CONCLUSIONS: l-lactic acid at physiological concentrations demonstrates potent HIV virucidal activity distinct from acidity alone and greater than acetic acid, suggesting a protective role in the sexual transmission of HIV.

AB - When Lactobacillus spp. dominate the vaginal microbiota of women of reproductive age they acidify the vagina to pH /=0.4 (w/w) l-lactic acid. At a typical vaginal pH of 3.8, l-lactic acid at 1 (w/w) more potently and rapidly inactivated HIVBa-L and HIV-1 transmitter/founder strains compared with 1 (w/w) acetic acid and with acidity alone, all adjusted to pH 3.8. A final concentration of 1 (w/w) l-lactic acid maximally inactivated HIVBa-L in the presence of cervicovaginal secretions and seminal plasma. The anti-HIV activity of l-lactic acid was pH dependent, being abrogated at neutral pH, indicating that its virucidal activity is mediated by protonated lactic acid and not the lactate anion. CONCLUSIONS: l-lactic acid at physiological concentrations demonstrates potent HIV virucidal activity distinct from acidity alone and greater than acetic acid, suggesting a protective role in the sexual transmission of HIV.

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