Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism

Francisco Javier Tovar-Lopez, Mahyar Nasabi, Vijay Sivan, Kashahyar Khoshmanesh, Shaun Jackson, Gary Rosengarten, Arnan Mitchell, Warwick S. Nesbitt

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

Abstract

This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages740-742
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 1 Jan 2012
EventInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012) - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012
Conference number: 16th

Conference

ConferenceInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)
Abbreviated titleMicroTAS 2012
CountryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Advective transport
  • Flow focusing
  • Multilayer soft-lithography
  • Platelet aggregation
  • Shear gradients

Cite this

Tovar-Lopez, F. J., Nasabi, M., Sivan, V., Khoshmanesh, K., Jackson, S., Rosengarten, G., ... Nesbitt, W. S. (2012). Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 740-742). Chemical and Biological Microsystems Society.
Tovar-Lopez, Francisco Javier ; Nasabi, Mahyar ; Sivan, Vijay ; Khoshmanesh, Kashahyar ; Jackson, Shaun ; Rosengarten, Gary ; Mitchell, Arnan ; Nesbitt, Warwick S. / Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 740-742
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abstract = "This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.",
keywords = "Advective transport, Flow focusing, Multilayer soft-lithography, Platelet aggregation, Shear gradients",
author = "Tovar-Lopez, {Francisco Javier} and Mahyar Nasabi and Vijay Sivan and Kashahyar Khoshmanesh and Shaun Jackson and Gary Rosengarten and Arnan Mitchell and Nesbitt, {Warwick S.}",
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Tovar-Lopez, FJ, Nasabi, M, Sivan, V, Khoshmanesh, K, Jackson, S, Rosengarten, G, Mitchell, A & Nesbitt, WS 2012, Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 740-742, International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012), Okinawa, Japan, 28/10/12.

Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism. / Tovar-Lopez, Francisco Javier; Nasabi, Mahyar; Sivan, Vijay; Khoshmanesh, Kashahyar; Jackson, Shaun; Rosengarten, Gary; Mitchell, Arnan; Nesbitt, Warwick S.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 740-742.

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

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AU - Sivan, Vijay

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AU - Jackson, Shaun

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AU - Mitchell, Arnan

AU - Nesbitt, Warwick S.

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N2 - This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.

AB - This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.

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Tovar-Lopez FJ, Nasabi M, Sivan V, Khoshmanesh K, Jackson S, Rosengarten G et al. Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 740-742