Abstract
This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 740-742 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 1 Jan 2012 |
| Event | International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012) - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 Conference number: 16th |
Conference
| Conference | International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012) |
|---|---|
| Abbreviated title | MicroTAS 2012 |
| Country | Japan |
| City | Okinawa |
| Period | 28/10/12 → 1/11/12 |
Keywords
- Advective transport
- Flow focusing
- Multilayer soft-lithography
- Platelet aggregation
- Shear gradients
Cite this
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Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism. / Tovar-Lopez, Francisco Javier; Nasabi, Mahyar; Sivan, Vijay; Khoshmanesh, Kashahyar; Jackson, Shaun; Rosengarten, Gary; Mitchell, Arnan; Nesbitt, Warwick S.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 740-742.Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review
TY - GEN
T1 - Vacuum flow focusing microfluidics to study blood cell dynamics under shear gradient aggregation mechanism
AU - Tovar-Lopez, Francisco Javier
AU - Nasabi, Mahyar
AU - Sivan, Vijay
AU - Khoshmanesh, Kashahyar
AU - Jackson, Shaun
AU - Rosengarten, Gary
AU - Mitchell, Arnan
AU - Nesbitt, Warwick S.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.
AB - This paper reports on the development of a microfluidic platform to study mass transport behaviour of blood cells at micro-scale stenosis where local strain-rate micro-gradients trigger platelet aggregation. We present the design, fabrication and blood testing of a microfluidic device that incorporates symmetric or asymmetric flow focusing using a single pressure driver to study the hemodynamic variables promoting platelet aggregation at defined micro-contraction geometries. The thickness of the different streams can be controlled by changing the hydraulic resistance of the inlet feeder channels and by controlling the hydrostatic pressure of the reservoirs. This platform will facilitate the study of the role of mass transport phenomena in platelet thrombus formation and will lead to a greater understanding of the mechanism(s) underlying shear-gradient dependent discoid platelet aggregation in the context of cardiovascular diseases such as acute coronary syndromes and ischemic stroke.
KW - Advective transport
KW - Flow focusing
KW - Multilayer soft-lithography
KW - Platelet aggregation
KW - Shear gradients
UR - http://www.scopus.com/inward/record.url?scp=84901792032&partnerID=8YFLogxK
M3 - Conference Paper
AN - SCOPUS:84901792032
SN - 9780979806452
SP - 740
EP - 742
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -