Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis

V. R. Harley; P. J. Hudson; B. E H Coupar; P. W. Selleck; H. Westbury; D. B. Boyle

doi:10.1007/BF01318362

Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis

V. R. Harley, P. J. Hudson, B. E H Coupar, P. W. Selleck, H. Westbury, D. B. Boyle

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9 Citations (Scopus)

Abstract

The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.

Original language	English
Pages (from-to)	133-141
Number of pages	9
Journal	Archives of Virology
Volume	113
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01318362
Publication status	Published - 1 Mar 1990
Externally published	Yes

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title = "Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis",

abstract = "The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.",

author = "Harley, {V. R.} and Hudson, {P. J.} and Coupar, {B. E H} and Selleck, {P. W.} and H. Westbury and Boyle, {D. B.}",

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T1 - Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene

T2 - potential use in diagnosis

AU - Harley, V. R.

AU - Hudson, P. J.

AU - Coupar, B. E H

AU - Selleck, P. W.

AU - Westbury, H.

AU - Boyle, D. B.

PY - 1990/3/1

Y1 - 1990/3/1

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AB - The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.

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Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis

Abstract

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