Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis

V. R. Harley, P. J. Hudson, B. E H Coupar, P. W. Selleck, H. Westbury, D. B. Boyle

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The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalArchives of Virology
Issue number1-2
Publication statusPublished - 1 Mar 1990
Externally publishedYes

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