Vaccine-induced protection against gastrointestinal bacterial infections in the absence of secretory antibodies

Tania K. Uren, Odilia L C Wijburg, Cameron Simmons, Finn Eirik Johansen, Per Brandtzaeg, Richard A. Strugnell

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Secretory IgA (SIgA) is widely held to be responsible for the defense of the mucosae against pathogenics and other potentially harmful agents. In this study, polymeric Ig receptor (pIgR) knockout mice, which lack secretory antibodies (SAb), were used to investigate the role of vaccine-elicited SAb in protection against gastrointestinal bacterial infections. An essential role for specific SAb in protection against Vibrio cholerae was evident from experiments showing that vaccinated pIgR-/- mice, but not vaccinated C57BL/6 mice, were susceptible to cholera toxin challenge. Vaccination of C57BL/6 mice with Salmonella typhimurium elicited strong antigen-specific, mucosal responses, which blocked in vitro invasion of epithelia. However, vaccinated C57BL/6 and pIgR-/- mice were equally resistant to challenge infection with virulent S. typhimurium. Finally, we investigated the importance of SIgA in protection against recurrent infections with Citrobacter rodentium. Although higher numbers of bacteria were detected early after challenge infection in feces of vaccinated pIgR-/- mice compared with vaccinated C57BL/6 mice, both mouse strains showed complete clearance after 9 days. These results suggested that, in immune animals, SIgA is crucial for the protection of gastrointestinal surfaces against secreted bacterial toxins, may inhibit early colonization by C. rodentium, but is not essential for protection against reinfection with S. typhimurium or C. rodentium.

Original languageEnglish
Pages (from-to)180-188
Number of pages9
JournalEuropean Journal of Immunology
Volume35
Issue number1
DOIs
Publication statusPublished - 1 Jan 2005

Keywords

  • Antibodies
  • Bacterial
  • Mucosa
  • Rodent
  • Vaccination

Cite this

Uren, Tania K. ; Wijburg, Odilia L C ; Simmons, Cameron ; Johansen, Finn Eirik ; Brandtzaeg, Per ; Strugnell, Richard A. / Vaccine-induced protection against gastrointestinal bacterial infections in the absence of secretory antibodies. In: European Journal of Immunology. 2005 ; Vol. 35, No. 1. pp. 180-188.
@article{496f082330404a5280cb9221c4483c0a,
title = "Vaccine-induced protection against gastrointestinal bacterial infections in the absence of secretory antibodies",
abstract = "Secretory IgA (SIgA) is widely held to be responsible for the defense of the mucosae against pathogenics and other potentially harmful agents. In this study, polymeric Ig receptor (pIgR) knockout mice, which lack secretory antibodies (SAb), were used to investigate the role of vaccine-elicited SAb in protection against gastrointestinal bacterial infections. An essential role for specific SAb in protection against Vibrio cholerae was evident from experiments showing that vaccinated pIgR-/- mice, but not vaccinated C57BL/6 mice, were susceptible to cholera toxin challenge. Vaccination of C57BL/6 mice with Salmonella typhimurium elicited strong antigen-specific, mucosal responses, which blocked in vitro invasion of epithelia. However, vaccinated C57BL/6 and pIgR-/- mice were equally resistant to challenge infection with virulent S. typhimurium. Finally, we investigated the importance of SIgA in protection against recurrent infections with Citrobacter rodentium. Although higher numbers of bacteria were detected early after challenge infection in feces of vaccinated pIgR-/- mice compared with vaccinated C57BL/6 mice, both mouse strains showed complete clearance after 9 days. These results suggested that, in immune animals, SIgA is crucial for the protection of gastrointestinal surfaces against secreted bacterial toxins, may inhibit early colonization by C. rodentium, but is not essential for protection against reinfection with S. typhimurium or C. rodentium.",
keywords = "Antibodies, Bacterial, Mucosa, Rodent, Vaccination",
author = "Uren, {Tania K.} and Wijburg, {Odilia L C} and Cameron Simmons and Johansen, {Finn Eirik} and Per Brandtzaeg and Strugnell, {Richard A.}",
year = "2005",
month = "1",
day = "1",
doi = "10.1002/eji.200425492",
language = "English",
volume = "35",
pages = "180--188",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "1",

}

Vaccine-induced protection against gastrointestinal bacterial infections in the absence of secretory antibodies. / Uren, Tania K.; Wijburg, Odilia L C; Simmons, Cameron; Johansen, Finn Eirik; Brandtzaeg, Per; Strugnell, Richard A.

In: European Journal of Immunology, Vol. 35, No. 1, 01.01.2005, p. 180-188.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Vaccine-induced protection against gastrointestinal bacterial infections in the absence of secretory antibodies

AU - Uren, Tania K.

AU - Wijburg, Odilia L C

AU - Simmons, Cameron

AU - Johansen, Finn Eirik

AU - Brandtzaeg, Per

AU - Strugnell, Richard A.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Secretory IgA (SIgA) is widely held to be responsible for the defense of the mucosae against pathogenics and other potentially harmful agents. In this study, polymeric Ig receptor (pIgR) knockout mice, which lack secretory antibodies (SAb), were used to investigate the role of vaccine-elicited SAb in protection against gastrointestinal bacterial infections. An essential role for specific SAb in protection against Vibrio cholerae was evident from experiments showing that vaccinated pIgR-/- mice, but not vaccinated C57BL/6 mice, were susceptible to cholera toxin challenge. Vaccination of C57BL/6 mice with Salmonella typhimurium elicited strong antigen-specific, mucosal responses, which blocked in vitro invasion of epithelia. However, vaccinated C57BL/6 and pIgR-/- mice were equally resistant to challenge infection with virulent S. typhimurium. Finally, we investigated the importance of SIgA in protection against recurrent infections with Citrobacter rodentium. Although higher numbers of bacteria were detected early after challenge infection in feces of vaccinated pIgR-/- mice compared with vaccinated C57BL/6 mice, both mouse strains showed complete clearance after 9 days. These results suggested that, in immune animals, SIgA is crucial for the protection of gastrointestinal surfaces against secreted bacterial toxins, may inhibit early colonization by C. rodentium, but is not essential for protection against reinfection with S. typhimurium or C. rodentium.

AB - Secretory IgA (SIgA) is widely held to be responsible for the defense of the mucosae against pathogenics and other potentially harmful agents. In this study, polymeric Ig receptor (pIgR) knockout mice, which lack secretory antibodies (SAb), were used to investigate the role of vaccine-elicited SAb in protection against gastrointestinal bacterial infections. An essential role for specific SAb in protection against Vibrio cholerae was evident from experiments showing that vaccinated pIgR-/- mice, but not vaccinated C57BL/6 mice, were susceptible to cholera toxin challenge. Vaccination of C57BL/6 mice with Salmonella typhimurium elicited strong antigen-specific, mucosal responses, which blocked in vitro invasion of epithelia. However, vaccinated C57BL/6 and pIgR-/- mice were equally resistant to challenge infection with virulent S. typhimurium. Finally, we investigated the importance of SIgA in protection against recurrent infections with Citrobacter rodentium. Although higher numbers of bacteria were detected early after challenge infection in feces of vaccinated pIgR-/- mice compared with vaccinated C57BL/6 mice, both mouse strains showed complete clearance after 9 days. These results suggested that, in immune animals, SIgA is crucial for the protection of gastrointestinal surfaces against secreted bacterial toxins, may inhibit early colonization by C. rodentium, but is not essential for protection against reinfection with S. typhimurium or C. rodentium.

KW - Antibodies

KW - Bacterial

KW - Mucosa

KW - Rodent

KW - Vaccination

UR - http://www.scopus.com/inward/record.url?scp=12344285940&partnerID=8YFLogxK

U2 - 10.1002/eji.200425492

DO - 10.1002/eji.200425492

M3 - Article

VL - 35

SP - 180

EP - 188

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 1

ER -