Vaccination against ovine footrot using a live bacterial vector to deliver basic protease antigen

Robert J. Moore, David J. Stewart, Kim Lund, Adrian L.M. Hodgson

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A strain of Corynebacterium pseudotuberculosis, designated Toxminus, that has been rationally attenuated by deletion of the phospholipase D gene, is being developed as a live vaccine vector for the delivery of veterinary vaccine antigens. In the present study a recombinant form of the basic protease gene of the ovine footrot causative bacterium, Dichelobacter nodosus, was introduced into the vector strain using the high copy number plasmid pEP2. This strain secreted the basic protease protein. Vaccination trials in sheep with the recombinant strain demonstrated that, although an IgG immune response was elicited, the animals were not protected from footrot following artificial challenge under pen conditions. Although the sheep were not protected there was evidence that the progression of the disease was slowed.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalFEMS Microbiology Letters
Issue number2
Publication statusPublished - 15 Jan 2001


  • Basic protease
  • Corynebacterium pseudotuberculosis
  • Dichelobacter nodosus
  • Footrot
  • Vaccine vector

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