TY - JOUR
T1 - Utility of peptide nucleic acid fluorescence in situ hybridization for rapid detection of Acinetobacter spp. and Pseudomonas aeruginosa
AU - Peleg, Anton Yariv
AU - Tilahun, Y
AU - Fiandaca, M J
AU - D'Agata, E MC
AU - Venkataraman, L
AU - Moellering Jr, Robert C
AU - Eliopoulos, George M
PY - 2009
Y1 - 2009
N2 - The utility of peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Acinetobacter spp. and Pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of ATCC strains and clinical isolates with select gram-negative rods. After testing 60 clinical isolates, PNA FISH had a sensitivity and specificity of 100 and 100 , respectively, for Acinetobacter spp. and 100 and 95 , respectively, for P. aeruginosa. PNA FISH was able to detect both pathogens simultaneously and directly from spiked blood cultures.
AB - The utility of peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Acinetobacter spp. and Pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of ATCC strains and clinical isolates with select gram-negative rods. After testing 60 clinical isolates, PNA FISH had a sensitivity and specificity of 100 and 100 , respectively, for Acinetobacter spp. and 100 and 95 , respectively, for P. aeruginosa. PNA FISH was able to detect both pathogens simultaneously and directly from spiked blood cultures.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19116347
U2 - 10.1128/JCM.01724-08
DO - 10.1128/JCM.01724-08
M3 - Article
SN - 0095-1137
VL - 47
SP - 830
EP - 832
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 3
ER -