Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

In vivo visualization of the microvasculature of the mouse cremaster muscle has been fruitful in the evaluation of the role of macrophage migration inhibitory factor in promotion of leukocyte trafficking. Here we explain how to undertake this preparation, including details on mouse anesthesia, securing intravenous access, and cremaster muscle exteriorization. We also provide information on the various microscopy modalities now available for imaging microvascular preparations of this nature.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric F. Morand
PublisherHumana Press
Chapter3
Pages27-37
Number of pages11
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adhesion
  • Cremaster muscle
  • Intravital microscopy
  • Leukocyte interactions
  • Postcapillary venule
  • Rolling
  • Transmigration

Cite this

Norman, M. U., & Hickey, M. J. (2020). Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo. In J. Harris, & E. F. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 27-37). (Methods in molecular biology ; Vol. 2080). Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_3