@inbook{cf448bbd7bf6434f92617e5f4fa75a40,
title = "Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity During Legionella pneumophila Infection",
abstract = "Legionella pneumophila is an intracellular bacterial pathogen that uses a type IV secretion system (T4SS), termed Dot/Icm, to secrete more than 330 virulence effector proteins into the infected host cell. Many Dot/Icm effectors are involved in biogenesis of the Legionella-containing vacuole (LCV), which allows intracellular bacterial replication in environmental amoebae and alveolar macrophages. Through their activity, some effectors trigger the mammalian host immune response in a phenomenon termed effector-triggered immunity (ETI). Here, we describe a protocol to create and use L. pneumophila genome deletion mutants to identify effector(s) that alter pro-inflammatory cytokine production and bacterial clearance in the lungs of mice.",
keywords = "Effector-triggered immunity, In vivo infection, Legionella pneumophila, Mutagenesis, T4SS",
author = "Wibawa, \{Rachelia R.\} and Pengfei Li and Kathleen McCaffrey and Hartland, \{Elizabeth L.\}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2449-4\_3",
language = "English",
isbn = "9781071624487",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "23--41",
editor = "Kufer, \{Thomas A.\} and Maria Kaparakis-Liaskos",
booktitle = "Effector-Triggered Immunity",
address = "United States of America",
}