Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

Darshita Budhadev; Karinna Saxby; Julia Walton; Gideon Davies; Peter C Tyler; Ralf Schwörer; Martin A Fascione

doi:10.1039/c8ob02756k

Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

Darshita Budhadev, Karinna Saxby, Julia Walton, Gideon Davies, Peter C Tyler, Ralf Schwörer, Martin A Fascione

Research output: Contribution to journal › Article › Research › peer-review

22 Citations (Scopus)

Abstract

Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer™, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.

Original language	English
Pages (from-to)	1817-1821
Number of pages	5
Journal	Organic & Biomolecular Chemistry
Volume	17
Issue number	7
DOIs	https://doi.org/10.1039/c8ob02756k
Publication status	Published - 1 Jan 2019
Externally published	Yes

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abstract = "Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer{\texttrademark}, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.",

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AU - Budhadev, Darshita

AU - Saxby, Karinna

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AU - Davies, Gideon

AU - Tyler, Peter C

AU - Schwörer, Ralf

AU - Fascione, Martin A

PY - 2019/1/1

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AB - Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer™, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.

UR - https://www.scopus.com/pages/publications/85061493530

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Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

Abstract

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