Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

Darshita Budhadev; Karinna Saxby; Julia Walton; Gideon Davies; Peter C Tyler; Ralf Schwörer; Martin A Fascione

doi:10.1039/c8ob02756k

Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

Darshita Budhadev, Karinna Saxby, Julia Walton, Gideon Davies, Peter C Tyler, Ralf Schwörer, Martin A Fascione

Research output: Contribution to journal › Article › Research › peer-review

18 Citations (Scopus)

Abstract

Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer™, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.

Original language	English
Pages (from-to)	1817-1821
Number of pages	5
Journal	Organic & Biomolecular Chemistry
Volume	17
Issue number	7
DOIs	https://doi.org/10.1039/c8ob02756k
Publication status	Published - 1 Jan 2019
Externally published	Yes

Access to Document

10.1039/c8ob02756k

Cite this

@article{636a179ab00b4d2f84e372961e57f015,

title = "Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors",

abstract = "Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer{\texttrademark}, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.",

author = "Darshita Budhadev and Karinna Saxby and Julia Walton and Gideon Davies and Tyler, {Peter C} and Ralf Schw{\"o}rer and Fascione, {Martin A}",

year = "2019",

month = jan,

day = "1",

doi = "10.1039/c8ob02756k",

language = "English",

volume = "17",

pages = "1817--1821",

journal = "Organic & Biomolecular Chemistry",

issn = "1477-0520",

publisher = "The Royal Society of Chemistry",

number = "7",

}

TY - JOUR

T1 - Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

AU - Budhadev, Darshita

AU - Saxby, Karinna

AU - Walton, Julia

AU - Davies, Gideon

AU - Tyler, Peter C

AU - Schwörer, Ralf

AU - Fascione, Martin A

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer™, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.

AB - Herein we report synthesis of complex heparan sulfate oligosaccharide precursors by automated glycan assembly using disaccharide donor building blocks. Rapid access to a hexasaccharide was achieved through iterative solid phase glycosylations on a photolabile resin using Glyconeer™, an automated oligosaccharide synthesiser, followed by photochemical cleavage and glycan purification using simple flash column chromatography.

UR - http://www.scopus.com/inward/record.url?scp=85061493530&partnerID=8YFLogxK

U2 - 10.1039/c8ob02756k

DO - 10.1039/c8ob02756k

M3 - Article

C2 - 30543331

AN - SCOPUS:85061493530

SN - 1477-0520

VL - 17

SP - 1817

EP - 1821

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

IS - 7

ER -

Using automated glycan assembly (AGA) for the practical synthesis of heparan sulfate oligosaccharide precursors

Abstract

Access to Document

Other files and links

Cite this