Abstract
| Original language | English |
|---|---|
| Title of host publication | Kidney Research |
| Subtitle of host publication | Experimental Protocols |
| Editors | T D Hewitson, E R Smith, S G Holt |
| Place of Publication | New York NY USA |
| Publisher | Humana Press |
| Pages | 67-79 |
| Number of pages | 13 |
| Edition | 2nd |
| ISBN (Electronic) | 978-1-4939-3353-2 |
| ISBN (Print) | 978-1-4939-3351-8 |
| DOIs | |
| Publication status | Published - 2016 |
Publication series
| Name | Methods in Molecular Biology:Springer Protocols |
|---|---|
| Publisher | Springer |
| Volume | 1397 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Cite this
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Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. / Bennett, Kevin M; Beeman, Scott C; Baldelomar, Edwin J; Zhang, Min; Wu, Teresa; Hann, Bradley D; Bertram, John F; Charlton, Jennifer R.
Kidney Research: Experimental Protocols. ed. / T D Hewitson; E R Smith; S G Holt. 2nd. ed. New York NY USA : Humana Press, 2016. p. 67-79 (Methods in Molecular Biology:Springer Protocols; Vol. 1397).Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review
TY - CHAP
T1 - Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI
AU - Bennett, Kevin M
AU - Beeman, Scott C
AU - Baldelomar, Edwin J
AU - Zhang, Min
AU - Wu, Teresa
AU - Hann, Bradley D
AU - Bertram, John F
AU - Charlton, Jennifer R
PY - 2016
Y1 - 2016
N2 - Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.
AB - Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.
UR - http://www.ncbi.nlm.nih.gov/pubmed/26676128
U2 - 10.1007/978-1-4939-3353-2_7
DO - 10.1007/978-1-4939-3353-2_7
M3 - Chapter (Book)
SN - 978-1-4939-3351-8
T3 - Methods in Molecular Biology:Springer Protocols
SP - 67
EP - 79
BT - Kidney Research
A2 - Hewitson, T D
A2 - Smith, E R
A2 - Holt, S G
PB - Humana Press
CY - New York NY USA
ER -