Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI

Kevin M Bennett, Scott C Beeman, Edwin J Baldelomar, Min Zhang, Teresa Wu, Bradley D Hann, John F Bertram, Jennifer R Charlton

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

5 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.
Original languageEnglish
Title of host publicationKidney Research
Subtitle of host publicationExperimental Protocols
EditorsT D Hewitson, E R Smith, S G Holt
Place of PublicationNew York NY USA
PublisherHumana Press
Pages67-79
Number of pages13
Edition2nd
ISBN (Electronic)978-1-4939-3353-2
ISBN (Print)978-1-4939-3351-8
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology:Springer Protocols
PublisherSpringer
Volume1397
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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