Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI

Kevin M Bennett; Scott C Beeman; Edwin J Baldelomar; Min Zhang; Teresa Wu; Bradley D Hann; John F Bertram; Jennifer R Charlton

doi:10.1007/978-1-4939-3353-2_7

Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI

Kevin M Bennett, Scott C Beeman, Edwin J Baldelomar, Min Zhang, Teresa Wu, Bradley D Hann, John F Bertram, Jennifer R Charlton

Anatomy & Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

5 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.

Original language	English
Title of host publication	Kidney Research
Subtitle of host publication	Experimental Protocols
Editors	T D Hewitson, E R Smith, S G Holt
Place of Publication	New York NY USA
Publisher	Humana Press
Pages	67-79
Number of pages	13
Edition	2nd
ISBN (Electronic)	978-1-4939-3353-2
ISBN (Print)	978-1-4939-3351-8
DOIs	https://doi.org/10.1007/978-1-4939-3353-2_7
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology:Springer Protocols
Publisher	Springer
Volume	1397
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Access to Document

10.1007/978-1-4939-3353-2_7

http://www.ncbi.nlm.nih.gov/pubmed/26676128

Cite this

Bennett, K. M., Beeman, S. C., Baldelomar, E. J., Zhang, M., Wu, T., Hann, B. D., Bertram, J. F., & Charlton, J. R. (2016). Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. In T. D. Hewitson, E. R. Smith, & S. G. Holt (Eds.), Kidney Research: Experimental Protocols (2nd ed., pp. 67-79). (Methods in Molecular Biology:Springer Protocols; Vol. 1397). Humana Press. https://doi.org/10.1007/978-1-4939-3353-2_7

Bennett, Kevin M ; Beeman, Scott C ; Baldelomar, Edwin J ; Zhang, Min ; Wu, Teresa ; Hann, Bradley D ; Bertram, John F ; Charlton, Jennifer R. / Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. Kidney Research: Experimental Protocols. editor / T D Hewitson ; E R Smith ; S G Holt. 2nd. ed. New York NY USA : Humana Press, 2016. pp. 67-79 (Methods in Molecular Biology:Springer Protocols).

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abstract = "Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.",

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Bennett, KM, Beeman, SC, Baldelomar, EJ, Zhang, M, Wu, T, Hann, BD, Bertram, JF & Charlton, JR 2016, Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. in TD Hewitson, ER Smith & SG Holt (eds), Kidney Research: Experimental Protocols. 2nd edn, Methods in Molecular Biology:Springer Protocols, vol. 1397, Humana Press, New York NY USA, pp. 67-79. https://doi.org/10.1007/978-1-4939-3353-2_7

Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. / Bennett, Kevin M; Beeman, Scott C; Baldelomar, Edwin J; Zhang, Min; Wu, Teresa; Hann, Bradley D; Bertram, John F; Charlton, Jennifer R.

Kidney Research: Experimental Protocols. ed. / T D Hewitson; E R Smith; S G Holt. 2nd. ed. New York NY USA : Humana Press, 2016. p. 67-79 (Methods in Molecular Biology:Springer Protocols; Vol. 1397).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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AU - Bennett, Kevin M

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AU - Charlton, Jennifer R

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AB - Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.

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Bennett KM, Beeman SC, Baldelomar EJ, Zhang M, Wu T, Hann BD et al. Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. In Hewitson TD, Smith ER, Holt SG, editors, Kidney Research: Experimental Protocols. 2nd ed. New York NY USA: Humana Press. 2016. p. 67-79. (Methods in Molecular Biology:Springer Protocols). https://doi.org/10.1007/978-1-4939-3353-2_7