Use of a lytic bacteriophage to control Salmonella Enteritidis in retail food

Tze Young Thung, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari Premarathne, Wei San Chang, Yuet Ying Loo, Yih Zhet Chin, Chee Hao Kuan, Chia Wanq Tan, Dayang Fredalina Basri, Che Wan Jasimah Wan Mohamed Radzi, Son Radu

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26 Citations (Scopus)

Abstract

A Salmonella Enteritidis lytic bacteriophage designated as SE07 was isolated from retail meat samples. Electron micrograph revealed that phage SE07 belonged to family Podoviridae. Phage SE07 was relatively stable at the temperature ranging from 28 to 65 °C. Furthermore, it exhibited remarkable pH stability (between pH 4.0–11.0). The effectiveness of phage SE07 was determined in different food matrices (fruit juice, fresh eggs, beef and chicken meat) experimentally contaminated with S. Enteritidis. A significant reduction of S. Enteritidis population (about 2 log cycles) was obtained in fruit juice and fresh eggs after incubation at 4 °C for 48 h. Furthermore, within the same period, bacterial population was reduced by 2.1 and 2.0 log cycles on the bacteriophage treated beef and chicken meat samples, respectively. In this study, the obtained data suggested that phage SE07 might have potential uses against Salmonella food-borne infections.

Original languageEnglish
Pages (from-to)222-225
Number of pages4
JournalLWT
Volume78
DOIs
Publication statusPublished - May 2017
Externally publishedYes

Keywords

  • Bacteriophage
  • Bio-control
  • Food
  • Isolation
  • Salmonella Enteritidis

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