Ursolic Acid: Efficacy and Stability as a Preservative Against Mold and Yeast in Poultry Feed

Lotte Geerlofs, Zhiyong He, Peter Xiao, Vivan Liu, Zhi Cheng Xiao

Research output: Contribution to journalArticleResearch


Consumers have been demanding safer alternatives for chemical preservatives. There are natural compounds that could serve as preservatives in the future. The objective of this study was to investigate the efficacy of ursolic acid, either alone or in combination with berberine, against mold and yeast in poultry feed. First, the stability of ursolic acid was tested using HPLC to determine the concentration of ursolic acid in the feed. Furthermore, ursolic acid was mixed into the feed at a 0.05g/kg concentration or in combination with berberine at a
concentration of 0.03g/kg berberine and 0.05g/kg ursolic acid. A solution containing mold and yeast was added to each bag of feed. The mold and yeast concentration were determined at different time points. Addition of 0.05g/kg of ursolic acid to the feed significantly inhibited the growth of mold but not the growth of yeast. When combined with 0.03g/kg berberine, 0.05g/kg ursolic acid significantly inhibited the growth of both mold and yeast.
Original languageEnglish
Pages (from-to)633-637
Number of pages5
JournalApproaches in Poultry, Dairy & Veterinary Sciences
Issue number3
Publication statusPublished - 16 Dec 2019
Externally publishedYes


  • Ursolic acid
  • natural preservative
  • antimicrobial
  • stability
  • efficacy

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