Ureaplasma colonization of amniotic fluid and efficacy of antenatal corticosteroids for preterm lung maturation in sheep

Timothy J M Moss, Ilias Nitsos, Christine L Knox, Graeme R Polglase, Suhas G Kallapur, Machiko Ikegami, Alan H Jobe, John P Newnham

Research output: Contribution to journalArticleResearchpeer-review

Abstract

OBJECTIVE: The objective of the study was to assess the efficacy of maternal betamethasone for improving preterm lung function in the presence of inflammation induced by amniotic fluid Ureaplasma colonization. STUDY DESIGN: Ewes bearing single fetuses were randomized to receive an intraamniotic injection of Ureaplasma parvum (serovar 6; 2 x 10(7) colony-forming units) or vehicle at 86 +/- 2 days of pregnancy (mean +/- SD: term is 150 days), followed by maternal intramuscular betamethasone (0.5 mg/kg) or saline, either 2 or 7 days before delivery of lambs at 123 +/- 1 d. RESULTS: Amniotic fluid interleukin-8 was elevated by ureaplasmas (P = .049) but unaffected by betamethasone. Lung inflammation induced by ureaplasmas was not affected by betamethasone. Lung compliance was increased by Ureaplasma colonization (P = .009) and betamethasone (P = .042), and effects were additive. Lung surfactant was increased by Ureaplasma colonization (P <.001) and betamethasone 7 days (P = .001), but not 2 days, before delivery. CONCLUSION: Inflammation improves preterm lung function because of increases in surfactant. Antenatal corticosteroids further augment lung function through an apparently independent mechanism.
Original languageEnglish
Pages (from-to)96.e1 - 96.e6
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume200
Issue number1
DOIs
Publication statusPublished - 2009

Cite this

Moss, Timothy J M ; Nitsos, Ilias ; Knox, Christine L ; Polglase, Graeme R ; Kallapur, Suhas G ; Ikegami, Machiko ; Jobe, Alan H ; Newnham, John P. / Ureaplasma colonization of amniotic fluid and efficacy of antenatal corticosteroids for preterm lung maturation in sheep. In: American Journal of Obstetrics and Gynecology. 2009 ; Vol. 200, No. 1. pp. 96.e1 - 96.e6.
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title = "Ureaplasma colonization of amniotic fluid and efficacy of antenatal corticosteroids for preterm lung maturation in sheep",
abstract = "OBJECTIVE: The objective of the study was to assess the efficacy of maternal betamethasone for improving preterm lung function in the presence of inflammation induced by amniotic fluid Ureaplasma colonization. STUDY DESIGN: Ewes bearing single fetuses were randomized to receive an intraamniotic injection of Ureaplasma parvum (serovar 6; 2 x 10(7) colony-forming units) or vehicle at 86 +/- 2 days of pregnancy (mean +/- SD: term is 150 days), followed by maternal intramuscular betamethasone (0.5 mg/kg) or saline, either 2 or 7 days before delivery of lambs at 123 +/- 1 d. RESULTS: Amniotic fluid interleukin-8 was elevated by ureaplasmas (P = .049) but unaffected by betamethasone. Lung inflammation induced by ureaplasmas was not affected by betamethasone. Lung compliance was increased by Ureaplasma colonization (P = .009) and betamethasone (P = .042), and effects were additive. Lung surfactant was increased by Ureaplasma colonization (P <.001) and betamethasone 7 days (P = .001), but not 2 days, before delivery. CONCLUSION: Inflammation improves preterm lung function because of increases in surfactant. Antenatal corticosteroids further augment lung function through an apparently independent mechanism.",
author = "Moss, {Timothy J M} and Ilias Nitsos and Knox, {Christine L} and Polglase, {Graeme R} and Kallapur, {Suhas G} and Machiko Ikegami and Jobe, {Alan H} and Newnham, {John P}",
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Ureaplasma colonization of amniotic fluid and efficacy of antenatal corticosteroids for preterm lung maturation in sheep. / Moss, Timothy J M; Nitsos, Ilias; Knox, Christine L; Polglase, Graeme R; Kallapur, Suhas G; Ikegami, Machiko; Jobe, Alan H; Newnham, John P.

In: American Journal of Obstetrics and Gynecology, Vol. 200, No. 1, 2009, p. 96.e1 - 96.e6.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Ureaplasma colonization of amniotic fluid and efficacy of antenatal corticosteroids for preterm lung maturation in sheep

AU - Moss, Timothy J M

AU - Nitsos, Ilias

AU - Knox, Christine L

AU - Polglase, Graeme R

AU - Kallapur, Suhas G

AU - Ikegami, Machiko

AU - Jobe, Alan H

AU - Newnham, John P

PY - 2009

Y1 - 2009

N2 - OBJECTIVE: The objective of the study was to assess the efficacy of maternal betamethasone for improving preterm lung function in the presence of inflammation induced by amniotic fluid Ureaplasma colonization. STUDY DESIGN: Ewes bearing single fetuses were randomized to receive an intraamniotic injection of Ureaplasma parvum (serovar 6; 2 x 10(7) colony-forming units) or vehicle at 86 +/- 2 days of pregnancy (mean +/- SD: term is 150 days), followed by maternal intramuscular betamethasone (0.5 mg/kg) or saline, either 2 or 7 days before delivery of lambs at 123 +/- 1 d. RESULTS: Amniotic fluid interleukin-8 was elevated by ureaplasmas (P = .049) but unaffected by betamethasone. Lung inflammation induced by ureaplasmas was not affected by betamethasone. Lung compliance was increased by Ureaplasma colonization (P = .009) and betamethasone (P = .042), and effects were additive. Lung surfactant was increased by Ureaplasma colonization (P <.001) and betamethasone 7 days (P = .001), but not 2 days, before delivery. CONCLUSION: Inflammation improves preterm lung function because of increases in surfactant. Antenatal corticosteroids further augment lung function through an apparently independent mechanism.

AB - OBJECTIVE: The objective of the study was to assess the efficacy of maternal betamethasone for improving preterm lung function in the presence of inflammation induced by amniotic fluid Ureaplasma colonization. STUDY DESIGN: Ewes bearing single fetuses were randomized to receive an intraamniotic injection of Ureaplasma parvum (serovar 6; 2 x 10(7) colony-forming units) or vehicle at 86 +/- 2 days of pregnancy (mean +/- SD: term is 150 days), followed by maternal intramuscular betamethasone (0.5 mg/kg) or saline, either 2 or 7 days before delivery of lambs at 123 +/- 1 d. RESULTS: Amniotic fluid interleukin-8 was elevated by ureaplasmas (P = .049) but unaffected by betamethasone. Lung inflammation induced by ureaplasmas was not affected by betamethasone. Lung compliance was increased by Ureaplasma colonization (P = .009) and betamethasone (P = .042), and effects were additive. Lung surfactant was increased by Ureaplasma colonization (P <.001) and betamethasone 7 days (P = .001), but not 2 days, before delivery. CONCLUSION: Inflammation improves preterm lung function because of increases in surfactant. Antenatal corticosteroids further augment lung function through an apparently independent mechanism.

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JF - American Journal of Obstetrics and Gynecology

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