Grb7 is an adapter protein found to be overexpressed in several breast and other cancer cell types along with ErbB2. Grb7 is normally an interaction partner with focal adhesion kinase and in cancer
cells also aberrantly interacts with ErbB2. It is thus implicated in the migratory and proliferative potential of cancer cells. Previous studies have shown that the phage display-derived cyclic nonphosphorylated
inhibitor peptide, G7-18NATE, when linked to PenetratinA?, is able to interfere with the interaction of Grb7 with its upstream binding partners and to impact on both cell migration and proliferation. Here we report the synthesis of a biotinylated G7-18NATE covalently attached to just
the last seven residues of PenetratinA? (G7-18NATE-P-Biotin). We demonstrate that this construct is taken up efficiently into MDA-MB-468 breast cancer cells and co-localises with Grb7 in the cytoplasm. We also used isothermal titration calorimetry to determine the binding affinity of G7-18NATE-P-Biotin to the Grb7-SH2 domain, and showed that it binds with micromolar affinity (Kd=14.4 i??M), similar to the affinity of G7-18NATE (Kd= 35.4 i??M). Together this shows that this shorter G7-18NATE-P-Biotin construct is suitable for further studies of the anti-proliferative and anti-migratory potential of this inhibitor.