Upregulation of RCAN1 causes Down syndrome-like immune dysfunction

Katherine Rosemary Martin, Daniel Stuart Layton, Natalie Louise Seach, Alicia Robyn Irene Corlett, Maria-Jose Barallobre, Maria Arbones, Richard Lennox Boyd, Bernadette Maree Scott, Melanie April Pritchard

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

BACKGROUND: People with Down syndrome (DS) are more susceptible to infections and autoimmune disease, but the molecular genetic basis for these immune defects remains undetermined. In this study, we tested whether increased expression of the chromosome 21 gene RCAN1 contributes to immune dysregulation. METHODS: We investigated the immune phenotype of a mouse model that overexpresses RCAN1. RCAN1 transgenic (TG) mice exhibit T cell abnormalities that bear a striking similarity to the abnormalities described in individuals with DS. RESULTS: RCAN1-TG mice display T cell developmental defects in the thymus and peripheral immune tissues. Thymic cellularity is reduced by substantial losses of mature CD4 and CD8 thymocytes and medullary epithelium. In peripheral immune organs T lymphocytes are reduced in number and exhibit reduced proliferative capacity and aberrant cytokine production. These T cell defects are stem cell intrinsic in that transfer of wild type bone marrow into RCAN1-TG recipients restored medullary thymic epithelium and T cell numbers in the thymus, spleen and lymph nodes. However, bone marrow transplantation failed to improve T cell function, suggesting an additional role for RCAN1 in the non-haemopoietic compartment. CONCLUSIONS: RCAN1 therefore facilitates T cell development and function, and when overexpressed, may contribute to immune dysfunction in DS.
Original languageEnglish
Pages (from-to)444 - 454
Number of pages11
JournalJournal of Medical Genetics
Volume50
Issue number7
DOIs
Publication statusPublished - 2013

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