Update on the tutorial for learning and teaching macromolecular crystallography

Annette Faust, Sandra Puehringer, Nora Darowski, Santosh Panjikar, Kay Diederichs, Uwe Mueller, Manfred S. Weiss

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Abstract

Two new experiments (single isomorphous replacement including anomalous-scattering effects and radiation damage-induced phasing) have been designed to complement the five experiments (sulfur single-wavelength anomalous diffraction, multiple-wavelength anomalous diffraction, molecular replacement, ion binding and ligand binding) of the first edition of the previously described tutorial for learning and teaching macromolecular crystallography [Faust, Panjikar, Mueller, Parthasarathy, Schmidt, Lamzin & Weiss (2008). J. Appl. Cryst. 41, 1161-1172]. Furthermore, the tutorial has been re-organized and in part re-written to reflect the comments and suggestions of users. The most significant overhaul was applied to the data-processing part of the tutorial. Nevertheless, the convenient features that all of the utilized proteins used are commercially available and that they can be easily and reproducibly crystallized and mounted for diffraction data collection have been retained. Also, for all of the seven experiments the raw diffraction images and the processed data are provided for illustrating and teaching the steps of data processing and structure determination.

Original languageEnglish
Pages (from-to)1230-1237
Number of pages8
JournalJournal of Applied Crystallography
Volume43
Issue number5 PART 2
DOIs
Publication statusPublished - 2010
Externally publishedYes

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