Untargeted metabolic profiling of Eucalyptus spp. leaf oils using comprehensive two-dimensional gas chromatography with high resolution mass spectrometry: expanding the metabolic coverage

Yong Foo Wong, Patrick Perlmutter, Philip Marriott

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

Introduction: Chromatography with mass spectrometry (MS) is a technique of choice for metabolomic analysis of plant extracts. Single dimension gas chromatography (1DGC) with MS leads to poorly resolved metabolites of complex Eucalyptus spp. leaf oil secondary metabolites and consequently limited metabolic coverage of secondary compounds. Multidimensional chromatography with high resolution MS can contribute to advances in this field. Objectives: Deeper insight into metabolite composition and variation for Eucalyptus spp. leaf oils through systematic untargeted metabolic profiling using comprehensive two-dimensional GC (GC × GC) with high resolution time of-flight MS (accTOFMS), using generalised processes for metabolite identification. Methods: GC × GC separation used cryogenic modulation,with standard length polar first dimension and short fast analysis non-polar 2D columns. Compound tentative identification incorporated 1D and 2D retention information, retention indices, mass spectrum matching, and accurate mass MS data. Global metabolic profiles were interpreted through 2D contour plots and chemometric analysis.Results: Strategies for metabolite screening and identification using GC × GC-accTOFMS were proposed. Considerably more components are detected and recognised than for 1DGC. Structured 2D molecular composition chromatographic patterns aid identification. ca. 400 metabolites were detected, 183 compounds were identified or tentatively identified, representing between 50.8–90.0% of the total ion count, comprising various chemical families. PCA revealed discriminating metabolites, allowing chemotaxonomic classification of species.Conclusion: Expansion of metabolic coverage by using GC × GC-accTOFMS, and detailed 2D metabolic fingerprints of E. polybractea, E. citriodora, E. radiata and E. globulus leaf oils were established. This high resolution analytical platform, and identification strategy can be adapted to metabolic analysis of other plant extracts.
Original languageEnglish
Article number46
Number of pages17
JournalMetabolomics
Volume13
Issue number5
DOIs
Publication statusPublished - 1 May 2017

Keywords

  • Eucalyptus species
  • GC × GC
  • Accurate mass time-of-flight mass spectrometry
  • Metabolic coverage
  • Secondary metabolites

Cite this

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title = "Untargeted metabolic profiling of Eucalyptus spp. leaf oils using comprehensive two-dimensional gas chromatography with high resolution mass spectrometry: expanding the metabolic coverage",
abstract = "Introduction: Chromatography with mass spectrometry (MS) is a technique of choice for metabolomic analysis of plant extracts. Single dimension gas chromatography (1DGC) with MS leads to poorly resolved metabolites of complex Eucalyptus spp. leaf oil secondary metabolites and consequently limited metabolic coverage of secondary compounds. Multidimensional chromatography with high resolution MS can contribute to advances in this field. Objectives: Deeper insight into metabolite composition and variation for Eucalyptus spp. leaf oils through systematic untargeted metabolic profiling using comprehensive two-dimensional GC (GC × GC) with high resolution time of-flight MS (accTOFMS), using generalised processes for metabolite identification. Methods: GC × GC separation used cryogenic modulation,with standard length polar first dimension and short fast analysis non-polar 2D columns. Compound tentative identification incorporated 1D and 2D retention information, retention indices, mass spectrum matching, and accurate mass MS data. Global metabolic profiles were interpreted through 2D contour plots and chemometric analysis.Results: Strategies for metabolite screening and identification using GC × GC-accTOFMS were proposed. Considerably more components are detected and recognised than for 1DGC. Structured 2D molecular composition chromatographic patterns aid identification. ca. 400 metabolites were detected, 183 compounds were identified or tentatively identified, representing between 50.8–90.0{\%} of the total ion count, comprising various chemical families. PCA revealed discriminating metabolites, allowing chemotaxonomic classification of species.Conclusion: Expansion of metabolic coverage by using GC × GC-accTOFMS, and detailed 2D metabolic fingerprints of E. polybractea, E. citriodora, E. radiata and E. globulus leaf oils were established. This high resolution analytical platform, and identification strategy can be adapted to metabolic analysis of other plant extracts.",
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Untargeted metabolic profiling of Eucalyptus spp. leaf oils using comprehensive two-dimensional gas chromatography with high resolution mass spectrometry : expanding the metabolic coverage. / Wong, Yong Foo; Perlmutter, Patrick; Marriott, Philip.

In: Metabolomics, Vol. 13, No. 5, 46, 01.05.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Untargeted metabolic profiling of Eucalyptus spp. leaf oils using comprehensive two-dimensional gas chromatography with high resolution mass spectrometry

T2 - expanding the metabolic coverage

AU - Wong, Yong Foo

AU - Perlmutter, Patrick

AU - Marriott, Philip

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Introduction: Chromatography with mass spectrometry (MS) is a technique of choice for metabolomic analysis of plant extracts. Single dimension gas chromatography (1DGC) with MS leads to poorly resolved metabolites of complex Eucalyptus spp. leaf oil secondary metabolites and consequently limited metabolic coverage of secondary compounds. Multidimensional chromatography with high resolution MS can contribute to advances in this field. Objectives: Deeper insight into metabolite composition and variation for Eucalyptus spp. leaf oils through systematic untargeted metabolic profiling using comprehensive two-dimensional GC (GC × GC) with high resolution time of-flight MS (accTOFMS), using generalised processes for metabolite identification. Methods: GC × GC separation used cryogenic modulation,with standard length polar first dimension and short fast analysis non-polar 2D columns. Compound tentative identification incorporated 1D and 2D retention information, retention indices, mass spectrum matching, and accurate mass MS data. Global metabolic profiles were interpreted through 2D contour plots and chemometric analysis.Results: Strategies for metabolite screening and identification using GC × GC-accTOFMS were proposed. Considerably more components are detected and recognised than for 1DGC. Structured 2D molecular composition chromatographic patterns aid identification. ca. 400 metabolites were detected, 183 compounds were identified or tentatively identified, representing between 50.8–90.0% of the total ion count, comprising various chemical families. PCA revealed discriminating metabolites, allowing chemotaxonomic classification of species.Conclusion: Expansion of metabolic coverage by using GC × GC-accTOFMS, and detailed 2D metabolic fingerprints of E. polybractea, E. citriodora, E. radiata and E. globulus leaf oils were established. This high resolution analytical platform, and identification strategy can be adapted to metabolic analysis of other plant extracts.

AB - Introduction: Chromatography with mass spectrometry (MS) is a technique of choice for metabolomic analysis of plant extracts. Single dimension gas chromatography (1DGC) with MS leads to poorly resolved metabolites of complex Eucalyptus spp. leaf oil secondary metabolites and consequently limited metabolic coverage of secondary compounds. Multidimensional chromatography with high resolution MS can contribute to advances in this field. Objectives: Deeper insight into metabolite composition and variation for Eucalyptus spp. leaf oils through systematic untargeted metabolic profiling using comprehensive two-dimensional GC (GC × GC) with high resolution time of-flight MS (accTOFMS), using generalised processes for metabolite identification. Methods: GC × GC separation used cryogenic modulation,with standard length polar first dimension and short fast analysis non-polar 2D columns. Compound tentative identification incorporated 1D and 2D retention information, retention indices, mass spectrum matching, and accurate mass MS data. Global metabolic profiles were interpreted through 2D contour plots and chemometric analysis.Results: Strategies for metabolite screening and identification using GC × GC-accTOFMS were proposed. Considerably more components are detected and recognised than for 1DGC. Structured 2D molecular composition chromatographic patterns aid identification. ca. 400 metabolites were detected, 183 compounds were identified or tentatively identified, representing between 50.8–90.0% of the total ion count, comprising various chemical families. PCA revealed discriminating metabolites, allowing chemotaxonomic classification of species.Conclusion: Expansion of metabolic coverage by using GC × GC-accTOFMS, and detailed 2D metabolic fingerprints of E. polybractea, E. citriodora, E. radiata and E. globulus leaf oils were established. This high resolution analytical platform, and identification strategy can be adapted to metabolic analysis of other plant extracts.

KW - Eucalyptus species

KW - GC × GC

KW - Accurate mass time-of-flight mass spectrometry

KW - Metabolic coverage

KW - Secondary metabolites

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U2 - 10.1007/s11306-017-1173-3

DO - 10.1007/s11306-017-1173-3

M3 - Article

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VL - 13

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

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