TY - JOUR
T1 - Unravelling the Gastroprotective Potential of Kefir
T2 - Exploring Antioxidant Effects in Preventing Gastric Ulcers
AU - Côco, Larissa Zambom
AU - Aires, Rafaela
AU - Carvalho, Glaucimeire Rocha
AU - Belisário, Eduarda de Souza
AU - Yap, Michelle Khai Khun
AU - Amorim, Fernanda Gobbi
AU - Conde-Aranda, Javier
AU - Nogueira, Breno Valentim
AU - Vasquez, Elisardo Corral
AU - Pereira, Thiago de Melo Costa
AU - Campagnaro, Bianca Prandi
N1 - Funding Information:
This work was supported by Espírito Santo Research Foundation (FAPES) and by the National Council for the Development of Science and Technology (CNPq). FAPES supported BPC (Edital 21/2018—Universal: 120/2019; Edital 15/2022—PROFIX: 630/2022; Edital 02/2023—Taxa de Pesquisa: 317/2023) and TMCP (Edital 15/2022—PROFIX: 708/2022; Edital 20/2022—Chamada de Apoio a Núcleos Capixabas Emergentes em Pesquisa: 1042/2022). CNPq supported BPC (308220/2021-8), TMCP (309431/2022-0) and ECV (305740/2019-9). Xunta de Galicia: Plan Galego IDT, 2021–2022, M.C.-T., supported ECV (ED431B 2020/26e).
Publisher Copyright:
© 2023 by the authors.
PY - 2023/12
Y1 - 2023/12
N2 - The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-α levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1–35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-α systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.
AB - The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-α levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1–35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-α systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.
KW - anti-inflammatory
KW - bioprospection
KW - gastroprotection
KW - oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85180652512&partnerID=8YFLogxK
U2 - 10.3390/cells12242799
DO - 10.3390/cells12242799
M3 - Article
C2 - 38132119
AN - SCOPUS:85180652512
SN - 2073-4409
VL - 12
JO - Cells
JF - Cells
IS - 24
M1 - 2799
ER -