Unravelling the Gastroprotective Potential of Kefir: Exploring Antioxidant Effects in Preventing Gastric Ulcers

Larissa Zambom Côco, Rafaela Aires, Glaucimeire Rocha Carvalho, Eduarda de Souza Belisário, Michelle Khai Khun Yap, Fernanda Gobbi Amorim, Javier Conde-Aranda, Breno Valentim Nogueira, Elisardo Corral Vasquez, Thiago de Melo Costa Pereira, Bianca Prandi Campagnaro

Research output: Contribution to journalArticleResearchpeer-review


The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-α levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1–35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-α systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.

Original languageEnglish
Article number2799
Number of pages23
Issue number24
Publication statusPublished - Dec 2023


  • anti-inflammatory
  • bioprospection
  • gastroprotection
  • oxidative stress

Cite this