Understanding the crucial interactions between Cytochrome P450s and non-ribosomal peptide synthetases during glycopeptide antibiotic biosynthesis

Madeleine Peschke, Melanie Gonsior, Roderich D Sussmuth, Max J Cryle

Research output: Contribution to journalReview ArticleResearchpeer-review

30 Citations (Scopus)

Abstract

The importance of Cytochrome P450-catalyzed modifications of natural products produced by non-ribosomal peptide synthetase machineries is most apparent during glycopeptide antibiotic biosynthesis: specifically, the formation of essential amino acid side chains crosslinks in the peptide backbone of these clinically relevant antibiotics. These cyclization reactions take place whilst the peptide substrate remains bound to the non-ribosomal peptide synthetase in a process mediated by a conserved domain of previously unknown function - the X-domain. This review addresses recent advances in understanding P450 recruitment to non-ribosomal peptide synthetase-bound substrates and highlights the importance of both carrier proteins and the X-domain in different P450-catalyzed reactions.
Original languageEnglish
Pages (from-to)46-53
Number of pages8
JournalCurrent Opinion in Structural Biology
Volume41
DOIs
Publication statusPublished - 1 Dec 2016

Cite this

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abstract = "The importance of Cytochrome P450-catalyzed modifications of natural products produced by non-ribosomal peptide synthetase machineries is most apparent during glycopeptide antibiotic biosynthesis: specifically, the formation of essential amino acid side chains crosslinks in the peptide backbone of these clinically relevant antibiotics. These cyclization reactions take place whilst the peptide substrate remains bound to the non-ribosomal peptide synthetase in a process mediated by a conserved domain of previously unknown function - the X-domain. This review addresses recent advances in understanding P450 recruitment to non-ribosomal peptide synthetase-bound substrates and highlights the importance of both carrier proteins and the X-domain in different P450-catalyzed reactions.",
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Understanding the crucial interactions between Cytochrome P450s and non-ribosomal peptide synthetases during glycopeptide antibiotic biosynthesis. / Peschke, Madeleine; Gonsior, Melanie; Sussmuth, Roderich D; Cryle, Max J.

In: Current Opinion in Structural Biology, Vol. 41, 01.12.2016, p. 46-53.

Research output: Contribution to journalReview ArticleResearchpeer-review

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