Ultramarine, a chromoprotein acceptor for Forster resonance energy transfer

Anne Pettikiriarachchi, Lan Gong, Matthew A Perugini, Rodney J Devenish, Mark Prescott

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27 Citations (Scopus)

Abstract

We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; epsilon (585) (nm), 64,000 M(-1). cm(-1)) for use as a Forster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
Original languageEnglish
Article numbere41028
Number of pages9
JournalPLoS ONE
Volume7
Issue number7
DOIs
Publication statusPublished - 2012

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