TY - JOUR
T1 - Ultramarine, a chromoprotein acceptor for Forster resonance energy transfer
AU - Pettikiriarachchi, Anne
AU - Gong, Lan
AU - Perugini, Matthew A
AU - Devenish, Rodney J
AU - Prescott, Mark
PY - 2012
Y1 - 2012
N2 - We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; epsilon (585) (nm), 64,000 M(-1). cm(-1)) for use as a Forster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
AB - We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; epsilon (585) (nm), 64,000 M(-1). cm(-1)) for use as a Forster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
UR - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041028
U2 - 10.1371/journal.pone.0041028
DO - 10.1371/journal.pone.0041028
M3 - Article
SN - 1932-6203
VL - 7
JO - PLoS ONE
JF - PLoS ONE
IS - 7
M1 - e41028
ER -