Abstract
Background: The initiation of mechanical ventilation causes lung inflammation and injury with potential life-long consequences. Inert 50 nm polystyrene nanoparticles have been shown to reduce allergic inflammation in the lungs of adult mice. We aimed to investigate the potential
toxicity and prophylactic efficacy of nanoparticles to reduce ventilation induced lung injury (VILI).
Method: Preterm lambs (0.83 gestation) were delivered via caesarean section and either immediately euthanized (n = 5) or injuriously ventilated for 15 minutes with (n = 6) or without (n = 5) prophylactic nanoparticles (3% in 2 ml). Ventilation was subsequently continued for a total of 2 h to allow for manifestation of VILI. Lung tissue was snap frozen and collected for qrt-PCR analysis of proinflammatory cytokines and early injury gene marker expression.
Results: Injurious ventilation increased all markers of inflammation and injury compared to UVC. However, nanoparticle administration further increased proinflammatory cytokines (IL-1β, IL-6 and IL-8) and early lung injury genes (CTGF, ERG1 and CYR61) compared to lambs without
nanoparticles.
Conclusions: Prophylactic nanoparticle administration increased early
markers of lung inflammation and injury, indicating that at this dosage
inert 50 nm polystyrene nanoparticles are detrimental to the lung. Further
dosage studies are required to examine their efficacy for lung protection.
toxicity and prophylactic efficacy of nanoparticles to reduce ventilation induced lung injury (VILI).
Method: Preterm lambs (0.83 gestation) were delivered via caesarean section and either immediately euthanized (n = 5) or injuriously ventilated for 15 minutes with (n = 6) or without (n = 5) prophylactic nanoparticles (3% in 2 ml). Ventilation was subsequently continued for a total of 2 h to allow for manifestation of VILI. Lung tissue was snap frozen and collected for qrt-PCR analysis of proinflammatory cytokines and early injury gene marker expression.
Results: Injurious ventilation increased all markers of inflammation and injury compared to UVC. However, nanoparticle administration further increased proinflammatory cytokines (IL-1β, IL-6 and IL-8) and early lung injury genes (CTGF, ERG1 and CYR61) compared to lambs without
nanoparticles.
Conclusions: Prophylactic nanoparticle administration increased early
markers of lung inflammation and injury, indicating that at this dosage
inert 50 nm polystyrene nanoparticles are detrimental to the lung. Further
dosage studies are required to examine their efficacy for lung protection.
| Original language | English |
|---|---|
| Pages (from-to) | 7 |
| Number of pages | 1 |
| Journal | Journal of Paediatrics and Child Health |
| Volume | 55 |
| Issue number | Supp 1 |
| Publication status | Published - 2015 |
| Event | Annual Congress of the Perinatal-Society-of-Australia-and-New-Zealand 2015 - Crown Promenade, Melbourne, Australia Duration: 19 Apr 2015 → 22 Apr 2015 Conference number: 19th |