Ultra-high dose nanoparticles increased markers of lung injury and inflammation in the preterm lamb

Mikee Inocencio, Magdalena Plebanski, Sue Xiang, Mary Tolcos, Valerie Zahra, Tammy Lim, Domenic Anthony Larosa, Timothy Moss, Graeme Roger Polglase

Research output: Contribution to journalMeeting Abstractpeer-review


Background: The initiation of mechanical ventilation causes lung inflammation and injury with potential life-long consequences. Inert 50 nm polystyrene nanoparticles have been shown to reduce allergic inflammation in the lungs of adult mice. We aimed to investigate the potential
toxicity and prophylactic efficacy of nanoparticles to reduce ventilation induced lung injury (VILI).
Method: Preterm lambs (0.83 gestation) were delivered via caesarean section and either immediately euthanized (n = 5) or injuriously ventilated for 15 minutes with (n = 6) or without (n = 5) prophylactic nanoparticles (3% in 2 ml). Ventilation was subsequently continued for a total of 2 h to allow for manifestation of VILI. Lung tissue was snap frozen and collected for qrt-PCR analysis of proinflammatory cytokines and early injury gene marker expression.
Results: Injurious ventilation increased all markers of inflammation and injury compared to UVC. However, nanoparticle administration further increased proinflammatory cytokines (IL-1β, IL-6 and IL-8) and early lung injury genes (CTGF, ERG1 and CYR61) compared to lambs without
Conclusions: Prophylactic nanoparticle administration increased early
markers of lung inflammation and injury, indicating that at this dosage
inert 50 nm polystyrene nanoparticles are detrimental to the lung. Further
dosage studies are required to examine their efficacy for lung protection.
Original languageEnglish
Pages (from-to)7
Number of pages1
JournalJournal of Paediatrics and Child Health
Issue numberSupp 1
Publication statusPublished - 2015
EventAnnual Congress of the Perinatal-Society-of-Australia-and-New-Zealand 2015 - Crown Promenade, Melbourne, Australia
Duration: 19 Apr 201522 Apr 2015
Conference number: 19th

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